Fertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described. After gonadotropin stimulus, cumulus cells expand and form hyaluronan (HA)-rich cumulus ECM. In pigs, the proper structure of the cumulus ECM depends on the interaction between HA and serum-derived proteins of the inter-alpha-trypsin inhibitor (IαI) protein family. We have demonstrated the synthesis of HA by cumulus cells, and the presence of the IαI, tumor necrosis factor-alpha-induced protein 6 and pentraxin 3 in expanding oocyte-cumulus complexes (OCC). We have evaluated the covalent linkage of heavy chains of IαI proteins to HA, as the principal component of the expanded HA-rich cumulus ECM, in porcine OCC cultured in medium with specific inhibitors: AG1478 and lapatinib (both inhibitors of epidermal growth factor receptor tyrosine kinase activity); MG132 (a specific proteasomal inhibitor), indomethacin (cyclooxygenase inhibitor); and progesterone receptor antagonist (RU486). We have found that both RU486 and indomethacin does not disrupt the formation of the covalent linkage between the heavy chains of IαI to HA in the expanded OCC. In contrast, the inhibitors AG1478 and lapatinib prevent gonadotropin-induced cumulus expansion. Finally, the formation of oocyte-cumulus ECM relying on the covalent transfer of heavy chains of IαI molecules to HA has been inhibited in the presence of MG132.

• Keywords
• extracellular matrix, hyaluronan, inter-alpha-trypsin inhibitor, oocyte-cumulus complexes, pentraxin 3, tumor necrosis factor-alpha-induced protein 6,
• MeSH
• C-Reactive Protein metabolism   MeSH
• Extracellular Matrix drug effects   metabolism   MeSH
• Cumulus Cells cytology   drug effects   metabolism   MeSH
• Hyaluronic Acid metabolism   MeSH
• Mifepristone pharmacology   MeSH
• Cell Adhesion Molecules metabolism   MeSH
• Oocytes cytology   metabolism   MeSH
• Reproduction drug effects   MeSH
• Serum Amyloid P-Component metabolism   MeSH
• Animals MeSH
• Check Tag
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Review MeSH
• Names of Substances
• C-Reactive Protein MeSH
• Hyaluronic Acid MeSH
• Mifepristone MeSH
• Cell Adhesion Molecules MeSH
• PTX3 protein MeSH Browser
• Serum Amyloid P-Component MeSH
• Tnfaip6 protein, mouse MeSH Browser