Nejvíce citovaný článek - PubMed ID 24238285
Effect of chronic exposure to prometryne on oxidative stress and antioxidant response in common carp (Cyprinus carpio L.)
There is currently insufficient acknowledgment of the relationship between fish welfare and ultimate fillet quality. The purpose of this study was to assess the impacts of pre-slaughter handling and stocking density as fish welfare markers on fillet quality of largemouth bass (Micropterus salmoides). Fish from three stocking densities of 35, 50, and 65 kg·m-3 were reared in a recirculating aquaculture system (RAS) for 12 weeks and received commercial feed. Ultimately, the fish were either stunned with percussion on the head (control group) or subjected to air exposure for 3 min (anoxia group) before stunning and subsequent collection of blood and fillet samples. Western blot analysis revealed the degradation of actin in both groups. Additionally, higher oxidation progress and lower hardness and pH were observed in anoxia compared to the control group. We observed higher hardness at 35 kg·m-3 in anoxia compared to 50 and 65 km-3. The initial hardness values at 35, 50, and 65 km-3 were 1073, 841, and 813 (g) respectively in the anoxia group. Furthermore, the anoxia and control groups had rigor mortis after 6 and 10 h, respectively. Cortisol and glucose levels, and oxidative enzymes activity were higher in anoxia than in the control group. In conclusion, oxidation induced by anoxia likely plays a crucial role as a promoter of the quality deterioration of largemouth bass fillets.
- Klíčová slova
- anoxia, antioxidant capacity, blood biochemistry, oxidation development, welfare,
- Publikační typ
- časopisecké články MeSH
The sensitivity of early life stages of common carp (Cyprinus carpio L.) to chronic exposure to single and combined environmental concentrations of the triazine metabolites terbuthylazine 2-hydroxy, terbuthylazine-desethyl and atrazine 2-hydroxy was evaluated under laboratory conditions. Their effects were assessed on lipid peroxidation, antioxidant enzymes (total superoxide dismutase, glutathione reductase, catalase, glutathione S-transferase, reduced glutathione), mortality, growth, development and histology. Single metabolites (terbuthylazine 2-hydroxy-0.73 μg/L; terbuthylazine-desethyl-1.80 μg/L; atrazine 2-hydroxy-0.66 μg/L) and combinations were not associated with negative effects on hatching, behaviour, embryo viability, growth or early ontogeny. Carp exposed to terbuthylazine-desethyl at 1.80 μg/L showed significantly lower total superoxide dismutase and glutathione reductase activity compared with the control group. Liver histology revealed diffused steatosis associated with the presence of lipid inclusions in hepatic cells in groups exposed to terbuthylazine-desethyl, atrazine 2-hydroxy and the tested combination of metabolites.
- Klíčová slova
- Antioxidant enzymes, Atrazine 2-hydroxy, Fish, Terbuthylazine 2-hydroxy, Terbuthylazine-desethyl,
- MeSH
- antioxidancia metabolismus MeSH
- atrazin metabolismus toxicita MeSH
- chemické látky znečišťující vodu metabolismus toxicita MeSH
- embryo nesavčí účinky léků enzymologie metabolismus MeSH
- glutathion metabolismus MeSH
- glutathionreduktasa metabolismus MeSH
- glutathiontransferasa metabolismus MeSH
- herbicidy metabolismus toxicita MeSH
- játra účinky léků embryologie patologie MeSH
- kapři embryologie metabolismus MeSH
- katalasa metabolismus MeSH
- látky reagující s kyselinou thiobarbiturovou metabolismus MeSH
- oxidační stres účinky léků MeSH
- peroxidace lipidů účinky léků MeSH
- superoxiddismutasa metabolismus MeSH
- triaziny metabolismus toxicita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antioxidancia MeSH
- atrazin MeSH
- chemické látky znečišťující vodu MeSH
- glutathion MeSH
- glutathionreduktasa MeSH
- glutathiontransferasa MeSH
- herbicidy MeSH
- katalasa MeSH
- látky reagující s kyselinou thiobarbiturovou MeSH
- superoxiddismutasa MeSH
- terbutylazine MeSH Prohlížeč
- triaziny MeSH
The effects of Nexide (a.i. gamma-cyhalothrin 60 g L(-1)) on cumulative mortality, growth indices, and ontogenetic development of embryos and larvae of common carp (Cyprinus carpio L.) were studied. Levels of oxidative stress parameters glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), glutathione-S-transferase (GST), and lipid peroxidation were determined. Eggs of newly fertilised common carp were exposed to Nexide at concentrations 5, 25, 50, 100, and 250 μ g L(-1) (0.3, 1.5, 3, 6, and 15 μ g L(-1) gamma-cyhalothrin). All organisms exposed to concentrations higher than 50 μ g L(-1) died soon after hatching; at 25 μ g L(-1), 95% mortality was recorded. Larvae exposed to 5 μ g L(-1) showed significantly lower growth and retarded ontogenetic development compared to control. Histological examination of the livers of larvae from the exposed group revealed dystrophic changes. The value of detoxification enzyme GST of organisms from the exposed group was significantly higher compared to the control and the value of defensive enzyme GPx was significantly lower compared to the control. The results of our investigation confirmed that contamination of aquatic environment by pesticides containing cyhalothrin may impair growth and development of early life stages of carp and cause disbalance of defensive enzymes.
- MeSH
- chemické látky znečišťující vodu aplikace a dávkování toxicita MeSH
- embryonální vývoj účinky léků MeSH
- kapři embryologie růst a vývoj MeSH
- LD50 MeSH
- nitrily aplikace a dávkování toxicita MeSH
- oxidační stres účinky léků MeSH
- peroxidace lipidů účinky léků MeSH
- pesticidy toxicita MeSH
- přežití MeSH
- pyrethriny aplikace a dávkování toxicita MeSH
- stadia vývoje účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chemické látky znečišťující vodu MeSH
- cyhalothrin MeSH Prohlížeč
- nitrily MeSH
- pesticidy MeSH
- pyrethriny MeSH
The aim of the study was to investigate effects of the triazine herbicide prometryne on red swamp crayfish on the basis of oxidative stress, antioxidant indices in hepatopancreas and muscle, and histopathology of hepatopancreas. Crayfish were exposed to prometryne concentrations of 0.51 μ g L(-1), 0.144 mg L(-1), and 1.144 mg L(-1) for 11 and 25 days. Indices of oxidative stress (thiobarbituric acid reactive substances (TBARS)), and antioxidant parameters (superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR)) in crayfish muscle and hepatopancreas were measured. Chronic exposure to prometryne did not showed the impact of oxidative damage to cells. Changes activity of the antioxidant enzymes SOD, CAT, and GR were observed in all tested concentrations to prometryne for 11 and 25 days (P < 0.01) as compared with the control group. We did not see any differences in histopatological examination to hepatopancreas. Prolonged exposure of prometryne did not result in oxidative damage to cell lipids and proteins, but it led to changes in antioxidant activity in crayfish tissues. Changes in antioxidant systems were also observed in the environmental prometryne concentration of 0.51 μ g L(-1). The results suggest that antioxidant responses may have potential as biomarkers for monitoring residual triazine herbicides in aquatic environments.
- MeSH
- antioxidancia metabolismus MeSH
- herbicidy škodlivé účinky farmakologie MeSH
- oxidační stres účinky léků MeSH
- oxidoreduktasy metabolismus MeSH
- prometryn škodlivé účinky farmakologie MeSH
- proteiny členovců metabolismus MeSH
- severní raci metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- herbicidy MeSH
- oxidoreduktasy MeSH
- prometryn MeSH
- proteiny členovců MeSH
The aim of the study was to investigate effects of the triazine's herbicide terbuthylazine-2-hydroxy on early life stage of common carp (Cyprinus carpio L.) through antioxidant indices, mortality, growth, development, and histopathology. Based on accumulated mortality in the experimental groups, lethal concentrations of terbuthylazine-2-hydroxy were estimated at 35-day LC50 = 10.9 mg/L terbuthylazine-2-hydroxy. By day 15, fish were exposed to 3.5 mg/L and by day 26, fish were exposed to 0.0029 mg/L; real environmental concentration in Czech rivers, 0.07 mg/L, 1.4 mg/L, and 3.5 mg/L terbuthylazine-2-hydroxy, showed significantly lower mass and total length compared with controls. Based on inhibition of growth in the experimental groups, lowest observed effect concentration (LOEC) = 0.002 mg/L terbuthylazine-2-hydroxy and no observed effect concentration (NOEC) = 0.0001 mg/L terbuthylazine-2-hydroxy. No significant negative effects on hatching or embryo viability were demonstrated at the concentrations tested, but significant differences in early ontogeny among groups were noted. Fish from the two highest tested concentrations showed a dose-related delay in development compared with the controls. Total superoxide dismutase (SOD) activity was significant lower in all groups testedly for terbuthylazine-2-hydroxy compared with the control group. At concentrations of 1.4 and 3.5 mg/L damage to caudal kidney tubules when compared to control fish was found.
- MeSH
- analýza přežití MeSH
- antioxidancia metabolismus MeSH
- embryo nesavčí účinky léků MeSH
- kapři anatomie a histologie embryologie růst a vývoj MeSH
- larva účinky léků MeSH
- lidé MeSH
- orgánová specificita účinky léků MeSH
- stadia vývoje účinky léků MeSH
- tělesná hmotnost účinky léků MeSH
- tělesná výška účinky léků MeSH
- testy toxicity MeSH
- triaziny toxicita MeSH
- životní prostředí * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- terbutylazine MeSH Prohlížeč
- triaziny MeSH