Potato (Solanum tuberosum) is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, and flooding, which are severely exacerbated by climate change. Our understanding of crop acclimation to abiotic stress, however, remains limited. Here, we present a comprehensive molecular and physiological high-throughput profiling of potato (Solanum tuberosum, cv. Désirée) under heat, drought, and waterlogging applied as single stresses or in combinations designed to mimic realistic future scenarios. Stress responses were monitored via daily phenotyping and multi-omics analyses of leaf samples comprising proteomics, targeted transcriptomics, metabolomics, and hormonomics at several timepoints during and after stress treatments. Additionally, critical metabolites of tuber samples were analyzed at the end of the stress period. We performed integrative multi-omics data analysis using a bioinformatic pipeline that we established based on machine learning and knowledge networks. Waterlogging produced the most immediate and dramatic effects on potato plants, interestingly activating ABA responses similar to drought stress. In addition, we observed distinct stress signatures at multiple molecular levels in response to heat or drought and to a combination of both. In response to all treatments, we found a downregulation of photosynthesis at different molecular levels, an accumulation of minor amino acids, and diverse stress-induced hormones. Our integrative multi-omics analysis provides global insights into plant stress responses, facilitating improved breeding strategies toward climate-adapted potato varieties.

• MeSH
• fenotyp MeSH
• fyziologický stres * genetika   MeSH
• hlízy rostlin MeSH
• listy rostlin fyziologie   MeSH
• metabolomika MeSH
• multiomika MeSH
• období sucha MeSH
• proteomika MeSH
• regulace genové exprese u rostlin MeSH
• Solanum tuberosum * fyziologie   genetika   metabolismus   MeSH
• transkriptom MeSH
• Publikační typ
• časopisecké články MeSH

Jasmonates are a family of oxylipin phytohormones regulating plant development and growth and mediating "defense versus growth" responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood. An isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected years ago in wounded leaves of flowering plants, opening up the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp accumulating in response to biotic and abiotic stress in Arabidopsis (Arabidopsis thaliana). The OPDA amino acid conjugates displayed cis-OPDA-related plant responses in a JA-Ile-dependent manner. We also showed that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are mediated by members of the amidosynthetase GRETCHEN HAGEN 3 and the amidohydrolase INDOLE-3-ACETYL-LEUCINE RESISTANT 1/ILR1-like families. Thus, OPDA amino acid conjugates function in the catabolism or temporary storage of cis-OPDA in stress responses instead of acting as chemical signals per se.

• MeSH
• amidy metabolismus   MeSH
• Arabidopsis * genetika   metabolismus   MeSH
• cyklopentany * metabolismus   MeSH
• fyziologický stres * MeSH
• homeostáza * MeSH
• isoleucin analogy a deriváty   metabolismus   MeSH
• nenasycené mastné kyseliny * metabolismus   MeSH
• oxylipiny * metabolismus   MeSH
• regulace genové exprese u rostlin MeSH
• regulátory růstu rostlin metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 12-oxophytodienoic acid MeSH Prohlížeč
• amidy MeSH
• cyklopentany * MeSH
• isoleucin MeSH
• jasmonic acid MeSH Prohlížeč
• nenasycené mastné kyseliny * MeSH
• oxylipiny * MeSH
• regulátory růstu rostlin MeSH

This article comments on: Vladimir Y. Gorshkov, Yana Y. Toporkova, Ivan D. Tsers, Elena O. Smirnova, Anna V. Ogorodnikova, Natalia E. Gogoleva, Olga I. Parfirova, Olga E. Petrova, and Yuri V. Gogolev, Differential modulation of the lipoxygenase cascade during typical and latent Pectobacterium atrosepticum infections, Annals of Botany, Volume 129, Issue 3, 16 Februray 2022, Pages 271–285 https://doi.org/10.1093/aob/mcab108

• Klíčová slova
• Lipoxygenase pathway, Pectobacterium atrosepticum, typical and latent infections,
• MeSH
• lipoxygenasa MeSH
• nemoci rostlin mikrobiologie   MeSH
• oxylipiny * MeSH
• Pectobacterium * MeSH
• Publikační typ
• komentáře MeSH
• práce podpořená grantem MeSH
• úvodníky MeSH
• Názvy látek
• lipoxygenasa MeSH
• oxylipiny * MeSH

Plant diseases pose a substantial threat to food availability, accessibility, and security as they account for economic losses of nearly $300 billion on a global scale. Although various strategies exist to reduce the impact of diseases, they can introduce harmful chemicals to the food chain and have an impact on the environment. Therefore, it is necessary to understand and exploit the plants' immune systems to control the spread of pathogens and enable sustainable agriculture. Recently, growing pieces of evidence suggest a functional myriad of lipids to be involved in providing structural integrity, intracellular and extracellular signal transduction mediators to substantial cross-kingdom cell signaling at the host-pathogen interface. Furthermore, some pathogens recognize or exchange plant lipid-derived signals to identify an appropriate host or development, whereas others activate defense-related gene expression. Typically, the membrane serves as a reservoir of lipids. The set of lipids involved in plant-pathogen interaction includes fatty acids, oxylipins, phospholipids, glycolipids, glycerolipids, sphingolipids, and sterols. Overall, lipid signals influence plant-pathogen interactions at various levels ranging from the communication of virulence factors to the activation and implementation of host plant immune defenses. The current review aims to summarize the progress made in recent years regarding the involvement of lipids in plant-pathogen interaction and their crucial role in signal transduction.

• Klíčová slova
• lipids, microbes, oxylipins, pathogens, phosphatidic acid, plants,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

: Jasmonic acid (JA) and its related derivatives are ubiquitously occurring compounds of land plants acting in numerous stress responses and development. Recent studies on evolution of JA and other oxylipins indicated conserved biosynthesis. JA formation is initiated by oxygenation of α-linolenic acid (α-LeA, 18:3) or 16:3 fatty acid of chloroplast membranes leading to 12-oxo-phytodienoic acid (OPDA) as intermediate compound, but in Marchantiapolymorpha and Physcomitrellapatens, OPDA and some of its derivatives are final products active in a conserved signaling pathway. JA formation and its metabolic conversion take place in chloroplasts, peroxisomes and cytosol, respectively. Metabolites of JA are formed in 12 different pathways leading to active, inactive and partially active compounds. The isoleucine conjugate of JA (JA-Ile) is the ligand of the receptor component COI1 in vascular plants, whereas in the bryophyte M. polymorpha COI1 perceives an OPDA derivative indicating its functionally conserved activity. JA-induced gene expressions in the numerous biotic and abiotic stress responses and development are initiated in a well-studied complex regulation by homeostasis of transcription factors functioning as repressors and activators.

• Klíčová slova
• JA biosynthetic enzymes, JA bypass, JA signaling, Jasmonic acid (JA) metabolites, active JA compounds, occurrence, transcription factors,
• MeSH
• chloroplasty metabolismus   MeSH
• cyklopentany metabolismus   MeSH
• druhová specificita MeSH
• kyselina alfa-linolenová metabolismus   MeSH
• Marchantia metabolismus   MeSH
• mastné kyseliny metabolismus   MeSH
• mechy metabolismus   MeSH
• metabolické sítě a dráhy MeSH
• nenasycené mastné kyseliny metabolismus   MeSH
• oxylipiny metabolismus   MeSH
• peroxizomy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• 12-oxophytodienoic acid MeSH Prohlížeč
• cyklopentany MeSH
• jasmonic acid MeSH Prohlížeč
• kyselina alfa-linolenová MeSH
• mastné kyseliny MeSH
• nenasycené mastné kyseliny MeSH
• oxylipiny MeSH