INTRODUCTION: Greenhouse tomato growers face the challenge of balancing fruit size and chemical quality traits. This study focused on elucidating the interplay between plant branching and light management on these traits, while maintaining consistent shoot density. METHODS: We evaluated one- and two-shoot plants under varying top light intensities using high-pressure sodium lamps and light-emitting diode (LED) inter-lighting. RESULTS: The reduced yield in the two-shoot plants was mainly due to smaller fruit size, but not due to source strength limitations, as evaluated through leaf weight ratio (LWR), chlorophyll index, specific leaf area (SLA), leaf dry matter percentage, and stem soluble carbohydrate accumulation. Enhanced lighting improved fruit weight and various fruit traits, such as dry matter content, total soluble carbohydrate content, and phenolic content, for both one- and two-shoot plant types. Despite lower mean fruit weight, two-shoot plants exhibited higher values for chemical fruit quality traits, indicating that the fruit growth of two-shoot plants is not limited by the available carbohydrates (source strength), but by the fruit sink strength. Diurnal analysis of fruit growth showed that two-shoot plants had reduced expansion during light transitions. This drop in fruit expansion was not related to changes in root pressure (measured as xylem sap exudation from decapitated plants), but might be related to diminished xylem area in the stem joint of the two-shoot plants. The concentration of several hormones, including cytokinins, was lower in two-shoot plants, suggesting a reduced fruit sink capacity. DISCUSSION: The predominant impact of branching to two-shoot plants on sink capacity suggests that the fruit growth is not limited by available carbohydrates (source strength). Alongside the observation that light supplementation and branching exert independent additive effects on fruit size and chemical traits, this illuminates the potential to independently regulate these aspects in greenhouse tomato production.

• Keywords
• branching, fruit growth, fruit quality, light-emitting diode, plant hormones, sink-source relationship, tomato,
• Publication type
• Journal Article MeSH

The objective of our study was to characterise the growth of tomato seedlings under various light spectra, but special attention has been paid to gaining a deeper insight into the details of photosynthetic light reactions. The following light combinations (generated by LEDs, constant light intensity at 300 μmol m-2 s-1) were used: blue/red light; blue/red light + far red; blue/red light + UV; white light that was supplemented with green, and white light that was supplemented with blue. Moreover, two combinations of white light for which the light intensity was changed by imitating the sunrise, sunset, and moon were also tested. The reference point was also light generated by high pressure sodium lamps (HPS). Plant growth/morphological parameters under various light conditions were only partly correlated with the photosynthetic efficiency of PSI and PSII. Illumination with blue/red as the main components had a negative effect on the functioning of PSII compared to the white light and HPS-generated light. On the other hand, the functioning of PSI was especially negatively affected under the blue/red light that was supplemented with FR. The FT-Raman studies showed that the general metabolic profile of the leaves (especially proteins and β-carotene) was similar in the plants that were grown under the HPS and under the LED-generated white light for which the light intensity changed during a day. The effect of various light conditions on the leaf hormonal balance (auxins, brassinosteroids) is also discussed.

• Keywords
• Solanum lycopersicum L., auxins, brassinosteroids, light spectral composition, photosynthesis, plant growth,
• MeSH
• Brassinosteroids metabolism   MeSH
• Chlorophyll metabolism   MeSH
• Photosynthesis * radiation effects   MeSH
• Photosystem I Protein Complex metabolism   radiation effects   MeSH
• Photosystem II Protein Complex metabolism   radiation effects   MeSH
• Indoleacetic Acids metabolism   MeSH
• Plant Leaves growth & development   metabolism   radiation effects   MeSH
• Metabolome MeSH
• Spectrum Analysis, Raman MeSH
• Plant Growth Regulators metabolism   MeSH
• Seedlings growth & development   metabolism   radiation effects   MeSH
• Solanum lycopersicum growth & development   metabolism   radiation effects   MeSH
• Light MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Brassinosteroids MeSH
• Chlorophyll MeSH
• Photosystem I Protein Complex MeSH
• Photosystem II Protein Complex MeSH
• Indoleacetic Acids MeSH
• Plant Growth Regulators MeSH

Continuous light (CL) or a predominant nitrogen supply as ammonium (NH4+) can induce leaf chlorosis and inhibit plant growth. The similarity in injuries caused by CL and NH4+ suggests involvement of overlapping mechanisms in plant responses to these conditions; however, these mechanisms are poorly understood. We addressed this topic by conducting full factorial experiments with tomato plants to investigate the effects of NO3- or NH4+ supply under diurnal light (DL) or CL. We used plants at ages of 26 and 15 days after sowing to initiate the treatments, and we modulated the intensity of the stress induced by CL and an exclusive NH4+ supply from mild to strong. Under DL, we also studied the effect of nitrogen (N) deficiency and mixed application of NO3- and NH4+. Under strong stress, CL and exclusive NH4+ supply synergistically inhibited plant growth and reduced chlorophyll content. Under mild stress, when no synergetic effect between CL and NH4+ was apparent on plant growth and chlorophyll content, we found a synergetic effect of CL and NH4+ on the accumulation of several plant stress hormones, with an especially strong effect for jasmonic acid (JA) and 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene, in xylem sap. This modulation of the hormonal composition suggests a potential role for these plant hormones in plant growth responses to the combined application of CL and NH4+. No synergetic effect was observed between CL and NH4+ for the accumulation of soluble carbohydrates or of mineral ions, indicating that these plant traits are less sensitive than the modulation of hormonal composition in xylem sap to the combined CL and NH4+ application. Under diurnal light, NH4+ did not affect the hormonal composition of xylem sap; however, N deficiency strongly increased the concentrations of phaseic acid (PA), JA, and salicylic acid (SA), indicating that decreased N concentration rather than the presence of NO3- or NH4+ in the nutrient solution drives the hormone composition of the xylem sap. In conclusion, N deficiency or a combined application of CL and NH4+ induced the accumulation of JA in xylem sap. This accumulation, in combination with other plant hormones, defines the specific plant response to stress conditions.

• Keywords
• abscisic acid, ammonium, jasmonic acid, nitrate, nitrogen, phytohormones, salicylic acid, tomato,
• Publication type
• Journal Article MeSH