Our previous studies have shown physiological and yield intensification of selected crops with the application of nanoparticles (NPs). However, the impact on the quantitative, qualitative, and yield parameters of maize (Zea mays L.) in field conditions remains highly debated. This study aimed to evaluate the effects of zinc oxide (ZnO-NPs), gold NPs anchored to meso-biosilica (Au-NP-bioSi), and titanium dioxide (TiO2-NPs) as biological stimulants under field conditions during the vegetation season of 2021 in the Central European region. The study assessed the effects on the number of plants, yield, yield components, and nutritional quality, including mineral nutrients, starch, and crude protein levels. The potential translocation of these chemically-physically stable NPs, which could pose a hazard, was also investigated. The results indicate that Au-NP-bioSi and ZnO-NPs-treatments were the most beneficial for yield and yield components at a statistically significant level. Mineral nutrient outcomes were varied, with the NP-free variant performing the best for phosphorus-levels, while Au-NP-bioSi and ZnO-NPs were optimal for crude protein. Starch content was comparable across the TiO2-NPs, Au-NP-bioSi, and control variants. Importantly, we observed no hazardous translocation of NPs or negative impacts on maize grain quality. This supports the hypothesis that NPs can serve as an effective tool for precise and sustainable agriculture.

• Klíčová slova
• biosilica, gold, grain quality, maize, nanofertilizers, nanoparticles, spray application, titanium dioxide, yield, zinc oxide,
• Publikační typ
• časopisecké články MeSH

In environmental and agronomic settings, even minor imbalances can trigger a range of unpredicted responses. Despite the widespread use of metal-based nanoparticles (NPs) and new bio-nanofertilizers, their impact on crop production is absent in the literature. Therefore, our research is focused on the agronomic effect of spray application of gold nanoparticles anchored to SiO2 mesoporous silica (AuSi-NPs), zinc oxide nanoparticles (ZnO-NPs), and iron oxide nanoparticles (Fe3O4-NPs) on sunflowers under real-world environments. Our findings revealed that the biosynthetically prepared AuSi-NPs and ZnO-NPs were highly effective in enhancing sunflower seasonal physiology, e.g., the value of the NDVI index increased from 0.012 to 0.025 after AuSi-NPs application. The distribution of leaf trichomes improved and the grain yield increased from 2.47 t ha-1 to 3.29 t ha-1 after ZnO-NPs application. AuSi-NPs treatment resulted in a higher content of essential linoleic acid (54.37%) when compared to the NPs-free control (51.57%), which had a higher determined oleic acid. No NPs or residual translocated metals were detected in the fully ripe sunflower seeds, except for slightly higher silica content after the AuSi-NPs treatment. Additionally, AuSi-NPs and NPs-free control showed wide insect biodiversity while ZnO-NPs treatment had the lowest value of phosphorus as anti-nutrient. Contradictory but insignificant effect on physiology, yield, and insect biodiversity was observed in Fe3O4-NPs treatment. Therefore, further studies are needed to fully understand the long-term environmental and agricultural sustainability of NPs applications.

• Klíčová slova
• foliar application, gold, insect biodiversity assessment, iron oxide, nanofertilizers, plant physiology, seeds quality, silica, sunflower yield, zinc,
• Publikační typ
• časopisecké články MeSH

In the 21st century, nanomaterials play an increasingly important role in our lives with applications in many sectors, including agriculture, biomedicine, and biosensors. Over the last two decades, extensive research has been conducted to find ways to synthesise nanoparticles (NPs) via mediation with fungi or fungal extracts. Mycosynthesis can potentially be an energy-efficient, highly adjustable, environmentally benign alternative to conventional physico-chemical procedures. This review investigates the role of metal toxicity in fungi on cell growth and biochemical levels, and how their strategies of resistance, i.e., metal chelation, biomineral formation, biosorption, bioaccumulation, compartmentalisation, and efflux of metals from cells, contribute to the synthesis of metal-containing NPs used in different applications, e.g., biomedical, antimicrobial, catalytic, biosensing, and precision agriculture. The role of different synthesis conditions, including that of fungal biomolecules serving as nucleation centres or templates for NP synthesis, reducing agents, or capping agents in the synthesis process, is also discussed. The authors believe that future studies need to focus on the mechanism of NP synthesis, as well as on the influence of such conditions as pH, temperature, biomass, the concentration of the precursors, and volume of the fungal extracts on the efficiency of the mycosynthesis of NPs.

• Klíčová slova
• biomolecule, biosynthesis, fungus, green synthesis, metal oxide nanoparticle, metallic nanoparticle, nanomaterial,
• MeSH
• bioakumulace MeSH
• biologický transport MeSH
• katalýza MeSH
• kovové nanočástice * MeSH
• redukční činidla MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• redukční činidla MeSH

Nanotechnologies have received tremendous attention since their discovery. The current studies show a high application potential of nanoparticles for plant treatments, where the general properties of nanoparticles such as their lower concentrations for an appropriate effects, the gradual release of nanoparticle-based nutrients or their antimicrobial effect are especially useful. The presented review, after the general introduction, analyzes the mechanisms that are described so far in the uptake and movement of nanoparticles in plants. The following part evaluates the available literature on the application of nanoparticles in the selective growth stage, namely, it compares the observed effect that they have when they are applied to seeds (nanopriming), to seedlings or adult plants. Based on the research that has been carried out, it is evident that the most common beneficial effects of nanopriming are the improved parameters for seed germination, the reduced contamination by plant pathogens and the higher stress tolerance that they generate. In the case of plant treatments, the most common applications are for the purpose of generating protection against plant pathogens, but better growth and better tolerance to stresses are also frequently observed. Hypotheses explaining these observed effects were also mapped, where, e.g., the influence that they have on photosynthesis parameters is described as a frequent growth-improving factor. From the consortium of the used nanoparticles, those that were most frequently applied included the principal components that were derived from zinc, iron, copper and silver. This observation implies that the beneficial effect that nanoparticles have is not necessarily based on the nutritional supply that comes from the used metal ions, as they can induce these beneficial physiological changes in the treated cells by other means. Finally, a critical evaluation of the strengths and weaknesses of the wider use of nanoparticles in practice is presented.

• Klíčová slova
• antibacterial effect, growth promotion, nanoparticles, nanopriming, seed germination, stress tolerance,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Nanotechnology offers new opportunities for the development of novel materials and strategies that improve technology and industry. This applies especially to agriculture, and our previous field studies have indicated that zinc oxide nanoparticles provide promising nano-fertilizer dispersion in sustainable agriculture. However, little is known about the precise ZnO-NP effects on legumes. Herein, 1 mg·L-1 ZnO-NP spray was dispersed on lentil plants to establish the direct NP effects on lentil production, seed nutritional quality, and stress response under field conditions. Although ZnO-NP exposure positively affected yield, thousand-seed weight and the number of pods per plant, there was no statistically significant difference in nutrient and anti-nutrient content in treated and untreated plant seeds. In contrast, the lentil water stress level was affected, and the stress response resulted in statistically significant changes in stomatal conductance, crop water stress index, and plant temperature. Foliar application of low ZnO-NP concentrations therefore proved promising in increasing crop production under field conditions, and this confirms ZnO-NP use as a viable strategy for sustainable agriculture.

• Klíčová slova
• essential and beneficial nutrients, foliar application, lentil seeds, nano-fertilizers, physiological indexes, zinc oxide nanoparticles,
• Publikační typ
• časopisecké články MeSH