Alizarin glucosyl transferase activity was found in five mutant strains of Streptomyces aureofaciens. The activity bears no direct relationship to the final products of tetracycline biosynthesis.

• MeSH
• anthrachinony farmakologie   MeSH
• glukosidy metabolismus   MeSH
• glukosyltransferasy izolace a purifikace   metabolismus   MeSH
• mutageneze MeSH
• Streptomyces aureofaciens enzymologie   genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alizarin MeSH Prohlížeč
• anthrachinony MeSH
• glukosidy MeSH
• glukosyltransferasy MeSH

Streptomyces aureofaciens glucosidizes 1,2,4-trihydroxy-9,10-anthraquinone (purpurin) added to the cultivation medium to yield the corresponding 2-beta-D-glucoside. The identity of the glucoside was demonstrated by comparing its physico-chemical properties with data of an authentic sample prepared synthetically. A further chemical glucosidation of the acetylated 2-beta-D-glucoside gives rise to 2-(hepta-O-acetyl-beta-gentiobiosyl)-4-(tetra-O-acetyl-beta-D-gluc opyranosyl) purpurin. All the derivatives are immunoactive.

• MeSH
• anthrachinony * MeSH
• chemické jevy MeSH
• chemie MeSH
• fermentace MeSH
• glukosidy biosyntéza   MeSH
• hmotnostní spektrometrie MeSH
• lektiny imunologie   metabolismus   MeSH
• magnetická rezonanční spektroskopie MeSH
• spektrofotometrie infračervená MeSH
• Streptomyces aureofaciens metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• anthrachinony * MeSH
• glukosidy MeSH
• lektiny MeSH
• purpurin anthraquinone MeSH Prohlížeč

Differential centrifugation, precipitation with ammonium sulphate and chromatography on DEAE-cellulose led to a twenty-fold purification of glucosyltransferase from Streptomyces aureofaciens B 96. The Michaelis constants for glucosyluridyl diphosphate (UDP-glucose) was 10.8 microM for 1,2-dihydroxy-9,10-anthraquinone (alizarin) 110 microM; the maximum rate of glucosylation reaction was 5.32 mumol per s per mg protein. The pH optimum was at 7.1; the flat temperature optimum was at 30 degrees C. Using some hydroxy derivatives of 9,10-anthraquinone it was found that the production of glucosides from aglycones with alpha-hydroxyl groups was about 1/8 of the values obtained with beta-hydroxyl substrates. In both types of aglycones the presence of another hydroxyl group led to a higher glucoside production.

• MeSH
• anthrachinony MeSH
• glukosidy MeSH
• glukosyltransferasy izolace a purifikace   metabolismus   MeSH
• koncentrace vodíkových iontů MeSH
• Streptomyces aureofaciens enzymologie   MeSH
• teplota MeSH
• uridindifosfátglukosa MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• anthrachinony MeSH
• glukosidy MeSH
• glukosyltransferasy MeSH
• uridindifosfátglukosa MeSH

Streptomyces aureofaciens B 96 grown on a synthetic medium glucosylated exogenous 1,2-dihydroxy-9,10-anthraquinone (alizarin). The glucosylation was inhibited by 2,4-dinitrophenol added to the cultivation medium. A cell-free preparation was obtained from the mycelium isolated after 16 h of growth and was found to catalyze the transfer of glucose from glucosyluridyl diphosphate to 1,2-dihydroxy-9,10-anthraquinone, giving rise to 1-hydroxy-2-(beta-D-glucopyranosyloxy)-9,10-anthraquinone.

• MeSH
• anthrachinony metabolismus   MeSH
• bakteriální proteiny biosyntéza   MeSH
• bezbuněčný systém MeSH
• dinitrofenoly farmakologie   MeSH
• glukosidy biosyntéza   MeSH
• glukosyltransferasy izolace a purifikace   metabolismus   MeSH
• sacharosa metabolismus   MeSH
• Streptomyces aureofaciens enzymologie   MeSH
• streptomycin farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• anthrachinony MeSH
• bakteriální proteiny MeSH
• dinitrofenoly MeSH
• glukosidy MeSH
• glukosyltransferasy MeSH
• sacharosa MeSH
• streptomycin MeSH