Monoclonal antibodies (mAb) to the transmissible gastroenteritis virus (TGEV) nucleoprotein (N) and membrane protein (M) were prepared and used for the comparative assessment of three blocking ELISA variants to detect TGEV. The competitive blocking ELISA format showed the highest sensitivity, allowing detection of 10(3) TCID50 TGEV/ml in culture medium. Ninety-nine porcine field faecal samples obtained from 37 herds affected with diarrhoea were examined, and various TGEV levels were found in nine samples from six herds. However, only in three samples were significant TGEV concentrations demonstrated. The relationship between incidence of TGEV gastroenteritis and the spread of porcine respiratory coronavirus infection in pig farms is discussed.

• MeSH
• antigeny virové analýza   MeSH
• ELISA metody   veterinární   MeSH
• feces virologie   MeSH
• monoklonální protilátky * imunologie   MeSH
• myši MeSH
• náhodné rozdělení MeSH
• novorozená zvířata MeSH
• nukleokapsida - proteiny imunologie   MeSH
• prasata MeSH
• přenosná gastroenteritida prasat diagnóza   MeSH
• proteiny virové matrix imunologie   MeSH
• protilátky virové imunologie   MeSH
• průjem diagnóza   veterinární   virologie   MeSH
• senzitivita a specificita MeSH
• virus přenosné gastroenteritidy prasat imunologie   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny virové MeSH
• monoklonální protilátky * MeSH
• nukleokapsida - proteiny MeSH
• proteiny virové matrix MeSH
• protilátky virové MeSH

Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.

• MeSH
• Coronavirus izolace a purifikace   MeSH
• elektroforéza v polyakrylamidovém gelu veterinární   MeSH
• elektronová mikroskopie veterinární   MeSH
• ELISA metody   veterinární   MeSH
• feces virologie   MeSH
• imunoenzymatické techniky veterinární   MeSH
• koronavirové infekce diagnóza   veterinární   virologie   MeSH
• monoklonální protilátky imunologie   MeSH
• nemoci prasat diagnóza   virologie   MeSH
• prasata MeSH
• proteiny virové matrix imunologie   MeSH
• protilátky blokující imunologie   MeSH
• průjem diagnóza   veterinární   virologie   MeSH
• senzitivita a specificita MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• monoklonální protilátky MeSH
• proteiny virové matrix MeSH
• protilátky blokující MeSH

Monoclonal antibodies to group A rotavirus Vp6 protein were prepared and used for verification of three blocking enzyme-linked immunosorbent assay (ELISA) modifications to detect rotavirus A. Selected competitive blocking ELISA (CB-ELISA) and electron microscopy (EM) were used for examination of 194 field faecal samples of piglets affected with diarrhoea. Rotavirus was detected in 43 samples (22.2%) by CB-ELISA method, whereas in 26 (13.4%) samples by EM examination. However, of 26 samples positive by EM, rotavirus A was detected by CB-ELISA in 19 (73.1%) samples; indicating the share of group A rotavirus in all cases of gastroenteritis caused by rotavirus. The sensitivity and specificity of the CB-ELISA was verified both by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhoea virus (PEDV) in each analysis and by comparative examination of samples with the commercial ELISA kit. The CB-ELISA sensitivity was positively affected by examination of samples in the presence of chelating agent.

• MeSH
• ELISA metody   veterinární   MeSH
• feces virologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• nemoci prasat diagnóza   virologie   MeSH
• prasata MeSH
• prediktivní hodnota testů MeSH
• protilátky virové analýza   MeSH
• rotavirové infekce diagnóza   veterinární   MeSH
• Rotavirus imunologie   izolace a purifikace   MeSH
• senzitivita a specificita MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• protilátky virové MeSH