Rous sarcoma virus-33 (RSV-33) was obtained from a sample of chicken Rous sarcoma which had been dried and stored in 1933. RSV-33, like the RSV-29, has the minimal number of passages beyond its isolation from chicken tumour No. 1. Our experiments demonstrated that the Rous sarcoma virus-33 was replication non-defective and was pathogenic for rats. Established rat tumorigenic cell lines express the viral genome. All three species of viral RNA were detected and v-src proteins and gag polyproteins were identified as well in cells of R9 and R74 lines. The virus can be rescued from cells of R9 and R74 lines, thus indicating that the cells are virogenic. The cells of a permanent tumorigenic line RT1 are infected but not transformed by RSV-33. Although they contain a complete proviral genome, they do not express detectable virus-specific RNA. The virus is not rescuable from RT1 cells under in vivo conditions. Proviral DNA analysis showed that the RSV-33 contained a full-length genome, including the env gene, in contrast to the RSV-29 which was found replication defective.
- MeSH
- buněčné linie MeSH
- chromozomy MeSH
- experimentální sarkom chemie mikrobiologie patologie MeSH
- genom virový MeSH
- krysa rodu Rattus MeSH
- kur domácí MeSH
- nádorové buňky kultivované MeSH
- potkani inbrední LEW MeSH
- proviry genetika MeSH
- virové proteiny metabolismus MeSH
- viry ptačího sarkomu růst a vývoj patogenita fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- virové proteiny MeSH
Increased synthesis of RecA protein is induced in E. coli cells after their damage, the rate of synthesis being dependent on the extent of DNA alterations. The level of the RecA protein was determined in E. coli cell extracts after damage induced by NQO, MNNG, MMC, NAL or UV radiation, using competitive enzyme-linked immunosorbent assay (ELISA). Purified E. coli RecA protein and rabbit monospecific polyclonal antibodies against it were prepared for the quantitative assay. The level of the RecA protein was increased after treatment with all mutagens. Contrary to other induced proteins, the synthesis of the RecA protein increased within 30 min after damage with UV radiation at a relatively slow rate. The ELISA method made it possible to determine 0.5-50 ng of the RecA protein in bacterial extracts. The method can be employed as an auxiliary test for DNA damage determination and also in studied concerning the role of the RecA protein in repair processes.
- MeSH
- ELISA MeSH
- Escherichia coli účinky léků metabolismus účinky záření MeSH
- mutageny MeSH
- poškození DNA fyziologie MeSH
- RecA-rekombinasy metabolismus MeSH
- ultrafialové záření MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mutageny MeSH
- RecA-rekombinasy MeSH
Pretreatment of MOLT-4 T-cell line with 12-0-tetradecanoylphorbol 13-acetate (TPA) potentiated the interferon alpha-induced cell surface expression of class I HLA. Simultaneous combined action of TPA and rIFN-alpha resulted in a synergistic effect upon the up-regulation of class I HLA. Neither TPA nor rIFN modulated the cell surface expression of HLA class II (DR and DP) antigens. Leucocyte common antigen (CD45) cell surface expression was quantitatively increased on MOLT-4 cells by TPA induction, unaltered by rIFN and up-regulated by combined action of TPA and rIFN. CD8 antigen was down-regulated by both TPA and rIFN.
- MeSH
- antigeny CD45 MeSH
- antigeny CD8 MeSH
- antigeny nádorové analýza MeSH
- antigeny povrchové analýza MeSH
- buněčné linie MeSH
- CD antigeny analýza MeSH
- diferenciační antigeny T-lymfocytů analýza MeSH
- down regulace účinky léků MeSH
- histokompatibilní antigeny analýza MeSH
- HLA antigeny analýza MeSH
- interferon alfa-2 MeSH
- interferon alfa farmakologie MeSH
- leukemie T-buněčná patologie MeSH
- lidé MeSH
- nádorové buňky kultivované účinky léků imunologie MeSH
- rekombinantní proteiny MeSH
- synergismus léků MeSH
- T-lymfocyty účinky léků imunologie MeSH
- tetradekanoylforbolacetát farmakologie MeSH
- upregulace účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny CD45 MeSH
- antigeny CD8 MeSH
- antigeny nádorové MeSH
- antigeny povrchové MeSH
- CD antigeny MeSH
- diferenciační antigeny T-lymfocytů MeSH
- histokompatibilní antigeny MeSH
- HLA antigeny MeSH
- interferon alfa-2 MeSH
- interferon alfa MeSH
- rekombinantní proteiny MeSH
- tetradekanoylforbolacetát MeSH
Apyrimidinic sites arising after excision of uracil incorporated into DNA daughter strands might constitute alkali-labile sites. The hypothesis was checked in the present paper and the data obtained do not support it.
- MeSH
- alkálie MeSH
- denaturace nukleových kyselin MeSH
- DNA bakterií chemie účinky záření MeSH
- Escherichia coli chemie účinky záření MeSH
- poškození DNA * MeSH
- pyrimidiny chemie MeSH
- replikace DNA MeSH
- ultrafialové záření MeSH
- vazebná místa MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alkálie MeSH
- DNA bakterií MeSH
- pyrimidiny MeSH
The possibility of expression of the gag gene of bovine leukaemia virus (BLV) in the bacterial system was investigated. The DNA fragment coding for the gag core 24 kDa protein of BLV was inserted into the pORF1 expression vector. The polypeptides expressed in E. coli were analysed by Western blotting. The bacterially synthesized antigens were detected by the serum of a BLV-infected cow and by mouse monoclonal antibodies against the native p24 gag protein.
- MeSH
- bakteriální geny MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- Escherichia coli genetika MeSH
- monoklonální protilátky MeSH
- peptidy analýza MeSH
- rekombinantní DNA MeSH
- Retroviridae genetika MeSH
- virové proteiny genetika metabolismus MeSH
- virus bovinní leukemie genetika MeSH
- western blotting MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- monoklonální protilátky MeSH
- peptidy MeSH
- rekombinantní DNA MeSH
- virové proteiny MeSH
An expression plasmid containing 1.224 bp of the bovine leukaemia virus (BLV) env gene was constructed. The polypeptides encoded by six recombinant plasmids were analysed by electrophoretic transfer blot analysis. Two new proteins of 150-160 kDa and 60 kDa, respectively, were found in whole cellular extracts using sera of naturally infected cattle and/or by use of mouse monoclonal antibodies against gp51.
- MeSH
- bakteriální geny MeSH
- Escherichia coli genetika MeSH
- ledviny cytologie MeSH
- ovce MeSH
- peptidy analýza MeSH
- plazmidy MeSH
- plod MeSH
- proteiny virového obalu genetika MeSH
- rekombinantní DNA MeSH
- Retroviridae genetika MeSH
- virus bovinní leukemie genetika MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- peptidy MeSH
- proteiny virového obalu MeSH
- rekombinantní DNA MeSH
Hybridoma cells were derived from a mouse immunized with freshly obtained primary human uveal melanoma cells. Supernatants from the resultant hybridoma clones were screened for positive antibody binding to uveal melanoma cell membranes and negative binding to membrane preparations of fibroblasts, retinal and uveal cells of healthy donors using sandwich radioimmunoassay and indirect immunofluorescence. Extensive specificity tests showed that the antibodies produced by ten clones bound strongly to fresh, and/or short-time cultivated primary human uveal melanoma tumour cells (UMEL-H, UMEL-K). Weaker binding occurred in a human uveal melanoma cell line (VUP-1), and/or in human skin melanoma cell lines (HMB-2, B-HM8). Binding assay of carcinoma cells, fibroblasts, uveal and retinal cells was negative. Intensive screening of this type is now under way.
- MeSH
- antigeny nádorové analýza MeSH
- antigeny povrchové analýza MeSH
- buněčné linie MeSH
- lidé MeSH
- melanom imunologie MeSH
- monoklonální protilátky * MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- nádory kůže imunologie MeSH
- nádory uvey imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny nádorové MeSH
- antigeny povrchové MeSH
- monoklonální protilátky * MeSH
A simple method of revealing proteins on nitrocellulose blots is described. It is based on high affinity of colloidal silver for proteins. The sensitivity is about the same as that of colloidal gold and it appears to be about 20 times higher than the presently used staining methods. The whole procedure is very simple and does not take more than 30 min.
Bovine leukaemia virus (BLV) propagated in a cell clone of foetal lamb kidney cells was transmitted by cell contact to bovine and ovine embryo cells derived from different organs. The transmission of the BLV genome was effectively achieved by cocultivation of mitomycin C-killed, virus-productive cells of the cell clone with embryo cells or by cell fusion. Infection of the same embryo cells by the virus was less effective. The transmission of BLV genome from nonproducer cells by the same procedure only exceptionally succeeded. The donor cells contained 3 integrated BLV proviruses. In the cells with transmitted BLV genome one provirus was found only. The majority of cells contained both unintegrated and integrated BLV provirus. In the cells containing the transmitted BLV, the virus genome was expressed to its protein products, some cells produced virus particles and reverse transcriptase activity into the medium. The implications of these findings are discussed with regard to biological and molecular properties of the retrovirus family to which the BLV belongs.
- MeSH
- buněčné linie MeSH
- DNA virů analýza MeSH
- embryo savčí cytologie MeSH
- fibroblasty mikrobiologie MeSH
- fúze buněk MeSH
- kultivované buňky MeSH
- ledviny cytologie MeSH
- ovce MeSH
- replikace viru MeSH
- Retroviridae - proteiny analýza MeSH
- Retroviridae fyziologie MeSH
- reverzní transkriptasa analýza MeSH
- virus bovinní leukemie izolace a purifikace fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA virů MeSH
- Retroviridae - proteiny MeSH
- reverzní transkriptasa MeSH
