Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo. Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases. The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites. GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line. The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays. The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well. Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect. The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion. The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.

• Klíčová slova
• Curcumin, Drug carrier, Inflammation, Monocytes, Pharmaceutical composite, β-Glucan microparticles,
• MeSH
• antiflogistika aplikace a dávkování   chemie   MeSH
• glukany aplikace a dávkování   chemie   MeSH
• interleukin-1beta metabolismus   MeSH
• kurkumin aplikace a dávkování   chemie   MeSH
• lidé MeSH
• lipopolysacharidy MeSH
• makrofágy účinky léků   metabolismus   MeSH
• monocyty účinky léků   metabolismus   MeSH
• NF-kappa B metabolismus   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• Saccharomyces cerevisiae MeSH
• THP-1 buňky MeSH
• TNF-alfa metabolismus   MeSH
• transkripční faktor AP-1 metabolismus   MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiflogistika MeSH
• glukany MeSH
• IL1B protein, human MeSH Prohlížeč
• interleukin-1beta MeSH
• kurkumin MeSH
• lipopolysacharidy MeSH
• NF-kappa B MeSH
• reaktivní formy kyslíku MeSH
• TNF protein, human MeSH Prohlížeč
• TNF-alfa MeSH
• transkripční faktor AP-1 MeSH

Yeast glucan particles (GPs) are promising agents for the delivery of biologically active compounds as drugs. GPs possess their own biological activities and can act synergistically with their cargo. This study aimed to determine how incorporating artemisinin, ellagic acid, (-)-epigallocatechin gallate, morusin, or trans-resveratrol into GPs affects their anti-inflammatory and antioxidant potential in vitro. Two different methods - slurry evaporation and spray drying - were used to prepare composites (GPs + bioactive compound) and the anti-inflammatory and antioxidative properties of the resultant products were compared. Several of the natural compounds showed the beneficial effects of being combined with GPs. The materials prepared by spray drying showed greater activity than those made using a rotary evaporator. Natural compounds incorporated into yeast GPs showed greater anti-inflammatory potential in vitro than simple suspensions of these compounds as demonstrated by their inhibition of the activity of transcription factors NF-κB/AP-1 and the secretion of the pro-inflammatory cytokine TNF-α.

• Klíčová slova
• Drug carrier, Inflammation, Monocytes, Natural compounds, Pharmaceutical composite, β-glucan microparticles,
• MeSH
• antiflogistika farmakologie   MeSH
• antioxidancia farmakologie   MeSH
• artemisininy farmakologie   MeSH
• cytokiny MeSH
• flavonoidy chemie   farmakologie   MeSH
• glukany chemie   farmakologie   MeSH
• katechin analogy a deriváty   chemie   farmakologie   MeSH
• kyselina ellagová chemie   farmakologie   MeSH
• monocyty účinky léků   MeSH
• NF-kappa B MeSH
• resveratrol chemie   farmakologie   MeSH
• Saccharomyces cerevisiae - proteiny MeSH
• Saccharomyces cerevisiae MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiflogistika MeSH
• antioxidancia MeSH
• artemisinin MeSH Prohlížeč
• artemisininy MeSH
• cytokiny MeSH
• epigallocatechin gallate MeSH Prohlížeč
• flavonoidy MeSH
• glukany MeSH
• katechin MeSH
• kyselina ellagová MeSH
• morusin MeSH Prohlížeč
• NF-kappa B MeSH
• resveratrol MeSH
• Saccharomyces cerevisiae - proteiny MeSH