• Klíčová slova
• CATALASE/urine *,
• MeSH
• katalasa moč   MeSH
• lidé MeSH
• tělesné tekutiny * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

• MeSH
• bakteriurie diagnóza   MeSH
• infekce močového ústrojí diagnóza   MeSH
• katalasa * MeSH
• katetrizace močového měchýře MeSH
• lidé MeSH
• metody MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa * MeSH

This study investigated the effect of one-week consumption of 165 g/day fresh blue honeysuckle berries (208 mg/day anthocyanins) in 10 healthy volunteers. At the end of intervention, levels of benzoic (median 1782 vs 4156), protocatechuic (709 vs 2417), vanillic (2779 vs 4753), 3-hydroxycinnamic (143 vs 351), p-coumaric (182 vs 271), isoferulic (805 vs 1570), ferulic (1086 vs 2395), and hippuric (194833 vs 398711 μg/mg creatinine) acids by LC/MS were significantly increased in the urine. Clinical chemistry safety markers were not altered. Oxidative stress markers, erythrocyte glutathione peroxidase (0.73 vs 0.88 U/g Hb) and catalase (2.5 vs 2.8 μkat/g Hb) activities, and erythrocyte/plasma thiobarbituric acid reactive substance (522 vs 612/33 vs 38 μmol/g Hb/protein) levels were significantly increased, without change in plasma antioxidant status. Nonsignificant changes of advanced oxidation protein products and oxidized LDL were observed. The results provide a solid base for further study of metabolite excretion and antioxidant parameters after ingestion of anthocyanins.

• MeSH
• anthokyaniny aplikace a dávkování   MeSH
• antioxidancia metabolismus   MeSH
• biologické markery moč   MeSH
• chromatografie kapalinová MeSH
• cinnamáty moč   MeSH
• dospělí MeSH
• erytrocyty metabolismus   MeSH
• glutathionperoxidasa krev   MeSH
• hippuráty moč   MeSH
• hmotnostní spektrometrie MeSH
• hydroxybenzoáty moč   MeSH
• katalasa krev   MeSH
• kyselina benzoová moč   MeSH
• kyselina vanilová moč   MeSH
• kyseliny kumarové moč   MeSH
• látky reagující s kyselinou thiobarbiturovou metabolismus   MeSH
• lidé MeSH
• lipoproteiny LDL krev   MeSH
• Lonicera chemie   MeSH
• metabolom * MeSH
• ovoce chemie   MeSH
• oxidační stres účinky léků   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anthokyaniny MeSH
• antioxidancia MeSH
• biologické markery MeSH
• cinnamáty MeSH
• glutathionperoxidasa MeSH
• hippuráty MeSH
• hippuric acid MeSH Prohlížeč
• hydroxybenzoáty MeSH
• isoferulic acid MeSH Prohlížeč
• katalasa MeSH
• kyselina benzoová MeSH
• kyselina vanilová MeSH
• kyseliny kumarové MeSH
• látky reagující s kyselinou thiobarbiturovou MeSH
• lipoproteiny LDL MeSH
• oxidized low density lipoprotein MeSH Prohlížeč
• phenolic acid MeSH Prohlížeč
• protocatechuic acid MeSH Prohlížeč

The study objective was to investigate the potential of a beverage containing silymarin and L-arginine to alter basic physiological and urodynamic parameters in 22 normal healthy men aged 38-59 years. The volunteers drank 500 ml/day beverage without silymarin and L-arginine for 10 days followed, after a 7-day washout period, by the beverage with 400mg silymarin and 295 mg L-arginine for 10 days. Blood and urine samples were collected on days 0, 10 and 27. The beverages were well-tolerated with no adverse effects. Most of the biochemical, hematological and urodynamic parameters remained unchanged. Total antioxidant capacity, total level of antioxidants, lipoperoxidation products (malondialdehyde), advanced oxidation products of proteins in plasma and glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase levels in erythrocytes were not influenced. Serum γ-glutamyl transferase, malondialdehyde level and activity of glutathione S-transferase in erythrocytes were lowered at day 27 and the concentration of total plasma SH-groups was higher on day 10. Using an ex vivo system, we found that silymarin/silybin at 10-100 μM is able to adsorb onto human erythrocytes and the complexes displayed antioxidant properties as studied using ex situ square-wave voltammetry. The trial showed that silymarin in vivo may protect erythrocytes against oxidative damage.

• MeSH
• antioxidancia aplikace a dávkování   MeSH
• arginin aplikace a dávkování   MeSH
• dospělí MeSH
• erytrocyty účinky léků   metabolismus   MeSH
• gama-glutamyltransferasa krev   MeSH
• glutathion krev   MeSH
• glutathionperoxidasa krev   MeSH
• glutathionreduktasa krev   MeSH
• glutathiontransferasa krev   MeSH
• katalasa krev   MeSH
• klinické křížové studie MeSH
• lidé středního věku MeSH
• lidé MeSH
• malondialdehyd krev   MeSH
• nápoje * MeSH
• pilotní projekty MeSH
• potravní doplňky * MeSH
• silibinin MeSH
• silymarin aplikace a dávkování   MeSH
• superoxiddismutasa krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• arginin MeSH
• gama-glutamyltransferasa MeSH
• glutathion MeSH
• glutathionperoxidasa MeSH
• glutathionreduktasa MeSH
• glutathiontransferasa MeSH
• katalasa MeSH
• malondialdehyd MeSH
• silibinin MeSH
• silymarin MeSH
• superoxiddismutasa MeSH

Cranberry (Vaccinium macrocarpon Ait. Ericaceae) fruits and juice are widely used for their antiadherence and antioxidative properties. Little is known however about their effects on clinical chemistry markers after long-term consumption. This study was conducted to evaluate the effect of three commercial cranberry products, NUTRICRAN90S, HI-PAC 4.0, and PACRAN on the antioxidative status of rodents, divided into three experimental groups. The products were given as dietary admixtures (1500 mg of product/kg of stock feed) for 14 weeks to male Wistar rats (Groups 2-4) and a control Group 1 which received only stock feed. There were no significant cranberry treatment-related effects on oxidative stress parameters, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase, total antioxidant capacity, thiobarbituric acid reactive substances, advanced oxidation protein products, total SH-groups, or any other measured clinical chemistry markers. Hematological parameters, body weight, and food consumption were also unaffected by intake of cranberries. Only liver glutathione reductase activity and glutathione levels were significantly lower in Group 4 than in Group 1. Plasma alkaline phosphatase alone was significantly decreased in Group 2. No gross pathology, effects on organ weights, or histopathology were observed. No genotoxicity was found, and total cytochrome P450 level in liver was unaffected in all groups. The levels of hippuric acid and several phenolic acids were significantly increased in plasma and urine in Groups 2-4. The concentration of anthocyanins was under the detection threshold. The dietary addition of cranberry powders for 14 weeks was well tolerated, but it did not improve the antioxidative status in rats.

• MeSH
• antioxidancia aplikace a dávkování   farmakologie   MeSH
• glutathionperoxidasa metabolismus   MeSH
• katalasa metabolismus   MeSH
• krysa rodu Rattus MeSH
• oxidační stres účinky léků   MeSH
• pilotní projekty MeSH
• potkani Wistar MeSH
• rostlinné přípravky aplikace a dávkování   farmakologie   MeSH
• Vaccinium macrocarpon chemie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• antioxidancia MeSH
• glutathionperoxidasa MeSH
• katalasa MeSH
• rostlinné přípravky MeSH

UVA photons are less energetic than UVB photons but they are more abundant in solar radiation. Modern tools have shown that UVA light has serious adverse effects on the skin. We investigated the effect of consuming Lonicera caerulea berries on UVA-induced damage in SKH-1 mice. The mice were fed a diet containing L. caerulea berries (10%, w/w) for 14 days before a single UVA (30 J/cm(2)) treatment. Effects on haematological and antioxidant parameters were evaluated 4 and 24h after irradiation. The bioavailability of L. caerulea phenolics was also assessed. Consuming the L. caerulea berry-enriched diet caused reduced malondialdehyde production and increased catalase activity and glutathione levels were found in skin and erythrocytes. UVA-induced NADPH:quinone oxidoreductase-1 and gamma-L-glutamate-L-cysteine ligase protein in skin were reduced in mice fed L. caerulea berries. Enhanced heme oxygenase-1 level in skin, interleukin-17 in plasma and reduced interleukin-12 levels in plasma were found in the mice on the experimental diet. Histological (pyknotic) changes in the nuclei of basal cells induced by UVA exposure were reduced in L. caerulea berry consuming animals. HLPC-MS analysis showed high concentrations of hippuric acid, one of the main metabolites of aromatic amino acids and phenolic compounds, in skin, liver, urine and faeces of mice consuming the berries. Taken together, consumption of L. caerulea berries affords protection from the adverse effects of a single UVA exposure mainly via modulation of antioxidant parameters.

• Klíčová slova
• Blue honeysuckle, Erythrocyte, Hairless mice, Liver, Skin, UVA radiation,
• MeSH
• antioxidancia metabolismus   MeSH
• dieta * MeSH
• enzymy metabolismus   MeSH
• erytrocyty metabolismus   účinky záření   MeSH
• glutathion metabolismus   MeSH
• hippuráty analýza   moč   MeSH
• interleukin-12 krev   MeSH
• interleukin-17 krev   MeSH
• játra chemie   MeSH
• kůže metabolismus   patologie   účinky záření   MeSH
• Lonicera chemie   metabolismus   MeSH
• malondialdehyd metabolismus   MeSH
• myši bezsrsté MeSH
• myši MeSH
• ovoce chemie   metabolismus   MeSH
• ultrafialové záření * MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• enzymy MeSH
• glutathion MeSH
• hippuráty MeSH
• hippuric acid MeSH Prohlížeč
• interleukin-12 MeSH
• interleukin-17 MeSH
• malondialdehyd MeSH

Nephrotoxicity as a cause of acute kidney injury (AKI) induced by cisplatin (CP), limits its usefulness as an anticancer agent. Diminazene, an angiotensin converting enzyme 2 activator, exhibited renoprotective properties on rat models of kidney diseases. This research aims to investigate the salutary effect of diminazene in comparison with lisinopril or valsartan in CP-induced AKI. The first and second groups of rats received oral vehicle (distilled water) for 9 days, and saline injection or intraperitoneal CP (6 mg/kg) on day 6, respectively. Third, fourth, and fifth groups received intraperitoneal injections of CP on day 6 and diminazene (15 mg/kg/day, orally), lisinopril (10 mg/kg/day, orally), or valsartan (30 mg/kg/day, orally), for 9 days, respectively. 24h after the last day of treatment, blood and kidneys were removed under anesthesia for biochemical and histopathological examination. Urine during the last 24 h before sacrificing the rats was also collected. CP significantly increased plasma urea, creatinine, neutrophil gelatinase-associated lipocalin, calcium, phosphorus, and uric acid. It also increased urinary albumin/creatinine ratio, N-Acetyl-beta-D-Glucosaminidase/creatinine ratio, and reduced creatinine clearance, as well the plasma concentrations of inflammatory cytokines [plasma tumor necrosis factor-alpha, and interleukin-1beta], and significantly reduced antioxidant indices [catalase, glutathione reductase , and superoxide dismutase]. Histopathologically, CP treatment caused necrosis of renal tubules, tubular casts, shrunken glomeruli, and increased renal fibrosis. Diminazine, lisinopril, and valsartan ameliorated CP-induced biochemical and histopathological changes to a similar extent. The salutary effect of the three drugs used is, at least partially, due to their anti-inflammatory and antioxidant effects. Keywords: Cisplatin, Diminazene, ACE2 activator, Lisinopril, Valsartan, Acute kidney injury.

• MeSH
• akutní poškození ledvin * chemicky indukované   patologie   metabolismus   prevence a kontrola   farmakoterapie   MeSH
• antitumorózní látky toxicita   MeSH
• cisplatina * toxicita   MeSH
• diminazen * analogy a deriváty   farmakologie   terapeutické užití   MeSH
• inhibitory ACE farmakologie   MeSH
• krysa rodu Rattus MeSH
• ledviny účinky léků   patologie   metabolismus   MeSH
• lisinopril * farmakologie   MeSH
• potkani Wistar * MeSH
• valsartan * farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• antitumorózní látky MeSH
• cisplatina * MeSH
• diminazen * MeSH
• inhibitory ACE MeSH
• lisinopril * MeSH
• valsartan * MeSH