Hydrogels based on natural and modified polysaccharides represent growing group of suitable matrices for the construction of effective wound healing materials. Bioactive tripeptide glycyl-l-histidyl-l-lysine and amino acid α-l-arginine are known to accelerate wound healing and skin repair. In this study, hydrogels based on low-methoxyl amidated citrus pectin or flaxseed gum were prepared and used for the transport of these healing agents to the experimental cutting wounds affected by extensive skin damage. Fourier-transform infrared spectroscopy, rheology, differential scanning calorimetry, scanning electron microscopy, swelling and release tests confirmed that these hydrogels differed in structure and physical properties. The cationic tripeptide was found to bind to carboxylic groups in LMA pectin, and the C3OH hydroxyl and ring oxygen O5 are involved in this interaction. The pectin hydrogel showed high viscosity and strong elastic properties, while the flaxseed gum hydrogel was characterised as a viscoelastic system of much lower viscosity. The former hydrogel released the drugs very slowly, while the latter hydrogel demonstrated zero order releasing kinetics optimal for drug delivery. In the in vivo wound healing testing on rats, both polysaccharide hydrogels improved the healing process mediated by the mentioned biomolecules. The tripeptide applied in the hydrogels showed significantly higher healing degree and lower healing time than in the control animals without treatment and when it was applied in an aqueous solution. Despite the absence of a synergistic effect, the mixture of the tripeptide and α-l-arginine in the hydrogels was also quite effective in wound healing. According to histological analysis, complete healing was achieved only when using the tripeptide in the flaxseed gum hydrogel. These observations might have an important prospect in clinical application of polysaccharide hydrogels.

• Klíčová slova
• Bioactive peptides, Flaxseed gum, Low methoxy amidated pectin, Polysaccharide hydrogels, Wound healing,
• MeSH
• gingiva chemie   MeSH
• hojení ran účinky léků   MeSH
• hydrogely chemie   farmakologie   MeSH
• krysa rodu Rattus MeSH
• kůže účinky léků   zranění   MeSH
• len chemie   MeSH
• lidé MeSH
• mikroskopie elektronová rastrovací MeSH
• oligopeptidy chemie   farmakologie   MeSH
• pektiny chemie   farmakologie   MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• citrus pectin MeSH Prohlížeč
• glycyl-histidyl-lysine MeSH Prohlížeč
• hydrogely MeSH
• oligopeptidy MeSH
• pektiny MeSH

Under certain conditions chondrocytes form lattices with cartilage collagens, which may serve as cartilage implants. It is necessary to find the optimal conditions for culturing chondrocytes. Three different supports are compared: (a) plastic; (b) cartilage collagens; and (c) insoluble skin collagen solubilized under denaturing conditions (ISC-40). The effect of culture medium supplementation with the tripeptide (Gly-His-Lys)2.Cu.2H2O.2NaCl (GHK) on chondrocyte proliferation and synthetic activity is studied, with particular attention paid to collagen types I, II and III. The collagen supports stimulated chondrocyte proliferation, but on the ISC-40 support they started to dedifferentiate rather early. In the primary culture, chondrocytes on all three supports synthesized mainly collagen type II, and only small amounts of types I and III. In the first passage the synthesis of these two collagen types increased, relative to collagen type II, at least on the cartilage collagen support. Supplementation of culture medium with GHK stimulated chondrocyte proliferation in the primary structure mostly on the ISC-40 support. On the other two types of supports the stimulatory effect of GHK was expressed mostly in the first passages. The collagen synthetic rate was increased by GHK on both of the collagen supports; on the cartilage collagen support collagen type II was synthesized predominantly and on the ISC-40 support types I and III were mostly formed. It is suggested that supplementation of culture medium with GHK may be useful in the preparation of cartilage implants.

• MeSH
• buněčné dělení účinky léků   MeSH
• chrupavka cytologie   účinky léků   metabolismus   MeSH
• denaturace proteinů MeSH
• DNA analýza   metabolismus   MeSH
• ELISA MeSH
• kolagen biosyntéza   chemie   MeSH
• kultivační média MeSH
• kultivované buňky MeSH
• kuřecí embryo MeSH
• kůže metabolismus   MeSH
• molekulární sekvence - údaje MeSH
• oligopeptidy farmakologie   MeSH
• plastické hmoty chemie   metabolismus   MeSH
• protézy a implantáty normy   MeSH
• růstové látky farmakologie   MeSH
• sekvence aminokyselin MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• kuřecí embryo MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA MeSH
• glycyl-histidyl-lysine MeSH Prohlížeč
• kolagen MeSH
• kultivační média MeSH
• oligopeptidy MeSH
• plastické hmoty MeSH
• růstové látky MeSH

The authors prepared 7.5% and 12.5% collagen gel, and supplemented it with the tripeptide Gly-His-Lys (GHK), perfloxacine and hypersulphated glycosaminoglycan (HSGAG). By means of 125l marking, its absorption was followed from small polyurethane sponges placed under the skin of rats. The absorption of gel without HSGAG was found to be faster. Antibodies against collagen (type I, II and III) or collagen gel were generated either in rabbits or in minipigs, in which collagen gel was tested. Microbiological tests proved the sterility of the collagen gel. The collagen gel supplemented with GHK, pefloxacine and HSGAG was named Colladel, and was used in a model experiment in guinea-pigs for filling artificially created bone defects in diaphyses of femurs, and with cementless endoprostheses. The healing process was followed by means of RTG and NMR, and histologically. The slowest healing process was found in unfilled bone defects. Defects filled with Colladel without GHK healed substantially more quickly, and the most accelerated healing was connected with complete Colladel application. When Colladel was used with cementless endoprostheses, vivid osteogenic activity at the interface of trabecular bone and metal stem was detectable in the course of the experiment.

• MeSH
• 4-chinolony * MeSH
• absorpce MeSH
• antiinfekční látky MeSH
• biodegradace MeSH
• biokompatibilní materiály * MeSH
• buňky pojivové tkáně MeSH
• chinolony MeSH
• femur MeSH
• fluorochinolony * MeSH
• gely MeSH
• glykosaminoglykany MeSH
• hojení ran * MeSH
• kolagen * imunologie   MeSH
• kosti a kostní tkáň * cytologie   MeSH
• králíci MeSH
• krysa rodu Rattus MeSH
• kůže MeSH
• kyčelní protézy * MeSH
• miniaturní prasata MeSH
• oligopeptidy * MeSH
• orchiektomie MeSH
• osteogeneze MeSH
• pefloxacin MeSH
• polyurethany * MeSH
• prasata MeSH
• protézy a implantáty * MeSH
• tvorba protilátek MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 4-chinolony * MeSH
• antiinfekční látky MeSH
• biokompatibilní materiály * MeSH
• chinolony MeSH
• fluorochinolony * MeSH
• gely MeSH
• glycyl-histidyl-lysine MeSH Prohlížeč
• glykosaminoglykany MeSH
• kolagen * MeSH
• oligopeptidy * MeSH
• polyurethany * MeSH