The study was based on a retrospective cephalometric assessment of 10-year-olds in order to evaluate the influence of early secondary bone grafting on craniofacial development in patients suffering from non-syndromic complete unilateral cleft lip and palate. The study consisted of 79 patients in the early and 67 patients in the late secondary bone grafting group. The mean age at alveolar bone grafting was 2.5 years (SD 0.03) in the first group and 9.8 years (SD 2.3) in the second group. The primary cleft repair of these 146 patients was always performed in accordance with the one-stage method. Additionally, the non-cleft Control group was comprised of 56 children of the same ethnicity and age. The cephalometric analysis performed at age 10 revealed similar overall characteristics of observed growth disturbances in both cleft groups in comparison to the Control group, such as: inhibition of vertical and anterior maxillary development, the tendency of the mandible to rotate clockwise, and a prevalence of vertical over horizontal facial growth. The comparison between the cleft groups revealed a lack of growth differences in the vertical dimension and more pronounced anterior maxillary development inhibition in the early bone grafting group. This study will be followed by a similar evaluation after craniofacial development is complete by a significant number of these patients in order to ascertain our conclusions.

• Klíčová slova
• Cephalometric, Cleft lip and palate, Early secondary bone grafting, Late secondary bone grafting, One-stage method,
• MeSH
• alveolární kostní štěp * metody   MeSH
• časové faktory MeSH
• dítě MeSH
• kefalometrie * MeSH
• lidé MeSH
• předškolní dítě MeSH
• retrospektivní studie MeSH
• rozštěp patra chirurgie   MeSH
• rozštěp rtu chirurgie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

OBJECTIVE: To evaluate the dental arch relationship in preadolescent children with complete unilateral cleft lip and palate after early secondary alveolar bone grafting (E-ABG) by comparing to late bone grafting (L-ABG). MATERIAL AND METHODS: Two raters blindly assessed the dental arch relationship with the modified Huddart-Bodenham (HB) Index for 2 groups: E-ABG group (36 children, mean age 9.6 years) and L-ABG group (56 children, mean age 11.1 years). The groups differed with respect to age at which alveolar bone grafting was performed: between 1.4 and 4.1 years (mean 2.2 years, E-ABG group) and after 8 years (L-ABG group). T-test was run to compare scores between 2 groups. Regression analysis was carried out to evaluate gender, age at cleft repair, age at ABG, and age at assessment with the HB index. RESULTS: The overall HB scores were -6.77 and -4.25 in the E-ABG and L-ABG groups, respectively (p = 0.025). Regression analysis showed that only the age at cleft repair influenced the HB scores. CONCLUSION: ABG carried out between 2 and 4 years of age does not seem to negatively affect the dental arch relationship at the age of 10 years in comparison to L-ABG.

• Klíčová slova
• Alveolar bone grafting, Cleft lip, Cleft palate, Dental arch relationship, One-stage repair,
• MeSH
• alveolární kostní štěp metody   MeSH
• dítě MeSH
• lidé MeSH
• rozštěp patra chirurgie   MeSH
• rozštěp rtu chirurgie   MeSH
• výsledek terapie MeSH
• zubní oblouk růst a vývoj   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Polsko MeSH

The study aimed at comparing maxillofacial morphology and burden of surgical treatment in post-pubertal patients with unilateral cleft lip and palate (UCLP) following early vs. late secondary alveolar bone grafting (SABG). In this retrospective study maxillofacial morphology was assessed on lateral cephalograms of skeletally matured patients with UCLP operated on consecutively in one center by the same method of one-stage primary cleft repair followed by SABG. Cephalometric analysis comprised 8 angular and 7 linear measurements. Maxillary growth restriction was evaluated by measuring SNA angle and Co-A and PNS-A distances. Five linear regression models with SNA, SNB, ANB, Co-A, and MP/SN as dependent variables and age at primary cleft repair, age at SABG, age at taking cephalogram, surgeon, and total number of surgical interventions as independent variables were also created. 135 patients were included in the study - 70 patients (47 males and 23 females) had early SABG (E-SABG group) and 65 patients (40 males and 25 females) had late SABG (L-SABG group). Mean age at bone grafting procedure was 2.7 years (range 1.5-5.9, SD 1.1) and 10.3 years (range 6.1-18.8, SD 3.2) in E-SABG and LSABG group respectively. The variables describing the degree of maxillary growth restriction - SNA, Co-A, and PNS-A - were comparable in both groups (p = 0.707, 0.116, and 0.932, respectively). Regression models demonstrated that independent variables were not associated with the values of SNA angle, SNB angle, ANB angle, MP/SN angle, and Co-A distance (p = 0.761, 0.088, 0.249, 0.380, and 0.363, respectively). The percentages of oronasal fistula repair, VPI repair, repeated SABG, upper lip correction, rhinoplasty, orthognathic surgery were 22.9, 7.1, 15.7, 24.3, 42.9, 2.9 in E-SABG group and 27.7, 7.7, 9.2, 36.9, 47.7, 3.1 in L-SABG group respectively. Mean number of all surgical interventions was 3.2 in E-SABG and 3.4 in L-SABG group, respectively (p = 0.271). Considering the comparable maxillofacial morphology and burden of surgical care in matured patients after early and late SABG, the choice of timing of bone grafting surgery could be left to the discretion of the surgeon.

• Klíčová slova
• Bone grafting, Cephalometric analysis, Maxillofacial morphology, Unilateral cleft lip and palate,
• MeSH
• alveolární kostní štěp * MeSH
• kefalometrie MeSH
• kojenec MeSH
• lidé MeSH
• předškolní dítě MeSH
• retrospektivní studie MeSH
• rozštěp patra * diagnostické zobrazování   chirurgie   MeSH
• rozštěp rtu * chirurgie   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Buried chip skin grafting used to close a granulation wound, that cannot be kept at rest, or else is in some way irritated, is described. This requires an extremely small amount of skin. Although it takes a month to heal, irritation or contamination of the grafted area does not affect or have any influence on the take of graft survival. Therefore, the patient need not be kept at rest after surgery, and can continue with daily living or rehabilitation exercises.

• MeSH
• dospělí MeSH
• hojení ran MeSH
• kožní vředy chirurgie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• pooperační komplikace chirurgie   MeSH
• popálení chirurgie   MeSH
• senioři MeSH
• transplantace kůže metody   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

This study evaluated the side-cleft grafting in native species of the genus Piper (Piper aduncum L., Piper hispidum Sw., and Piper tuberculatum Jacq.) with BRS Kottanadan pepper cultivar as rootstocks (Experiment 1). The top-cleft grafting was evaluated in native species Piper arboreum Aubl., P. aduncum, and P. tuberculatum with Balankotta pepper cultivar as graft using black pepper cv. Bragantina (Experiment 2). Black pepper cv. Bragantina autograft was considered as control. The rootstock P. tuberculatum showed initial incompatibility with the cv. Bragantina, with survival of about 34.8% (side) and 62.5% (top) and total inhibition of graft shooting. The side and top graft on P. aduncum resulted in 84.0 and 47.5% of survival, respectively. The JIP-test parameters indicated better photochemical efficiency in the species grafted using the method of side-cleft grafting. Finally, the initial compatibility was associated with greater survival, shooting, and better energy flow through the electron transport chain.

• Klíčová slova
• JIP test, grafting, pepper, propagation,
• Publikační typ
• časopisecké články MeSH

PURPOSE OF THE STUDY: The basic aim of the treatment of cystic nonunion is to provide stable fi xation and mechanical compression to increase union, but there is no consensus on whether to perform bone grafting in the cystic area or not. The aim of this study was to compare the clinical and radiological results of patients with cystic scaphoid non-union who received open grafting, and internal fi xation and those treated with percutaneous fi xation without grafting. MATERIAL AND METHODS A retrospective evaluation was made of patients included those determined radiologically with Slade and Dodds grade IV scaphoid cystic non-union. Two different surgical procedures were performed. Percutaneous screw fi xation was carried out on the patients in group 1. Open curettage-grafting and screw fi xation were performed on the patients in group 2. Group 1 was comprised of 16 patients treated with percutaneous screw fi xation, and group 2 was comprised of 17 patients who had open curettage-grafting and screw fi xation. RESULTS In the radiological evaluation at the fi nal follow-up examination, union was determined in 12 of the 16 (75%) patients in group 1 and 15 of the 17 (88%) patients in group 2. There was no statistically signifi cant difference between the two groups in terms of union, functional outcomes and complication rates. DISCUSSION In the current study, the union rate was higher in the patients who had open curettage-grafting and fi xation with a headless screw (88%) than in those with percutaneous screw without grafting (75%), but the difference was not determined to be statistically signifi cant. The union rate of the group that received grafting was similar to the rates reported in the literature. CONCLUSIONS We think that both of these techniques may be successfully used for cystic scaphoid nonunions but percutaneous technique should be preferred as it is minimally invasive. Nevertheless, there is a need for further prospective, randomised studies with larger series to have better comparisons. KEY WORDS: scaphoid bone, nonunion, fracture fi xation, internal, bone grafting, bone screws.

• MeSH
• člunkovitá kost * chirurgie   MeSH
• fraktury kostí * MeSH
• lidé MeSH
• pseudoartróza * chirurgie   MeSH
• retrospektivní studie MeSH
• transplantace kostí MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Early excision and grafting of the burn wound in the first 9 days remain the keys to survival for patients with major burn injuries. In the last 7-year-period, 54 major burn cases were treated in our burn facility, the only Burn Center in Istanbul. Early excision and grafting were performed to 32 of them, admitted in the first week. Others were admitted later and managed conservatively. Their follow-up results in terms of mortality and morbidity rates were compared. In addition to improvement in the prognosis, early excision and grafting procedures decreased the duration of hospitalization and cost of burn treatment.

• MeSH
• časové faktory MeSH
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• následné studie MeSH
• popálení komplikace   epidemiologie   chirurgie   MeSH
• popáleninové jednotky MeSH
• předškolní dítě MeSH
• revize kvality lékařské péče MeSH
• senioři MeSH
• transplantace kůže metody   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Turecko epidemiologie   MeSH

A novel concept based on advanced particle-grafting technology to tailor performance, damping, and surface properties of the magnetorheological elastomers (MREs) is introduced. In this work, the carbonyl iron (CI) particles grafted with poly(trimethylsilyloxyethyl methacrylate) (PHEMATMS) of two different molecular weights were prepared via surface-initiated atom transfer radical polymerization and the relations between the PHEMATMS chain lengths and the MREs properties were investigated. The results show that the magnetorheological performance and damping capability were remarkably influenced by different interaction between polydimethylsiloxane chains as a matrix and PHEMATMS grafts due to their different length. The MRE containing CI grafted with PHEMATMS of higher molecular weight exhibited a greater plasticizing effect and hence both a higher relative magnetorheological effect and enhanced damping capability were observed. Besides bulk MRE properties, the PHEMATMS modifications influenced also field-induced surface activity of the MRE sheets, which manifested as notable changes in surface roughness.

• Klíčová slova
• ATRP, damping, magnetorheology, polymer grafting, silicone elastomer, surface properties,
• Publikační typ
• časopisecké články MeSH

The development of a low-flow vascular prosthesis is a very topical issue. The authors present a pathway for the development of a prosthesis with optimal properties based on the idea of mimicking the characteristics of a biological model (saphenous vein graft) and programming these properties in the model of the prosthetic substitute. The vascular prosthesis presented consists of three layers - a non-absorbable scaffold representing vascular “media”, and two absorbable collagen layers - pseudointima and pseudoadventitia. The basic methods of physical testing are presented - the single axis stretch test and inflation-extension test, as well as other procedures that affect the final properties. These include collagen curing, antithrombotic treatment of the inner layer and the use of sterilization methods. The designed new graft was successfully implanted in an ovine model.

• Klíčová slova
• testing, vascular substitutes for low-flow peripheral bypass grafting,
• MeSH
• cévní protézy * MeSH
• krevní náhražky * MeSH
• lidé MeSH
• ovce MeSH
• průchodnost cév * MeSH
• vena saphena MeSH
• výkony cévní chirurgie * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• krevní náhražky * MeSH

The critical requirements in developing clinical-grade human-induced pluripotent stem cells-derived neural precursors (hiPSCs-NPCs) are defined by expandability, genetic stability, predictable in vivo post-grafting differentiation, and acceptable safety profile. Here, we report on the use of manual-selection protocol for generating expandable and stable human NPCs from induced pluripotent stem cells. The hiPSCs were generated by the reprogramming of peripheral blood mononuclear cells with Sendai-virus (SeV) vector encoding Yamanaka factors. After induction of neural rosettes, morphologically defined NPC colonies were manually harvested, re-plated, and expanded for up to 20 passages. Established NPCs showed normal karyotype, expression of typical NPCs markers at the proliferative stage, and ability to generate functional, calcium oscillating GABAergic or glutamatergic neurons after in vitro differentiation. Grafted NPCs into the striatum or spinal cord of immunodeficient rats showed progressive maturation and expression of early and late human-specific neuronal and glial markers at 2 or 6 months post-grafting. No tumor formation was seen in NPCs-grafted brain or spinal cord samples. These data demonstrate the effective use of in vitro manual-selection protocol to generate safe and expandable NPCs from hiPSCs cells. This protocol has the potential to be used to generate GMP (Good Manufacturing Practice)-grade NPCs from hiPSCs for future clinical use.

• Klíčová slova
• brain grafting, human-induced pluripotent stem cells (hiPSCs), immunodeficient rat, manual selection, neural precursor cells (NPCs), spinal cord grafting,
• MeSH
• buněčná diferenciace MeSH
• indukované pluripotentní kmenové buňky * MeSH
• krysa rodu Rattus MeSH
• leukocyty mononukleární MeSH
• lidé MeSH
• nervové kmenové buňky * MeSH
• neurony metabolismus   MeSH
• virus Sendai genetika   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH