This article gives a historical insight into the establishment of suitable models allowing the postulation that chicken Rous sarcoma virus (RSV) becomes integrated in different cells as a provirus. This is documented by the correspondence between two laboratories involved in these investigations. Special attention is paid to RSV-transformed mammalian cells, their virogenic nature, virus rescue by cell fusion, and finally their use for the oncogene v-src characterization. Two sets of experiments are mentioned, which provided an early indication of a transforming gene present in RSV.

• MeSH
• dějiny 20. století MeSH
• genom virový * MeSH
• krysa rodu Rattus MeSH
• kur domácí MeSH
• onkogenní viry genetika   růst a vývoj   MeSH
• proviry genetika   MeSH
• ptačí sarkom genetika   dějiny   virologie   MeSH
• reverzní transkriptasa genetika   dějiny   metabolismus   MeSH
• transformované buněčné linie MeSH
• virová transformace buněk genetika   MeSH
• viry ptačího sarkomu enzymologie   genetika   růst a vývoj   MeSH
• zvířata MeSH
• Check Tag
• dějiny 20. století MeSH
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• historické články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• reverzní transkriptasa MeSH

The aim of this work was to express the recombinant hexon protein of the hemorrhagic enteritis virus, to establish the diagnostic value of this protein for serological detection of antibodies in turkey serum samples and to assess seroprevalence of the infection in the Czech Republic. The N' terminal part of the hexon protein was expressed in a bacterial expression system and used as an antigen in an ELISA test for the detection of hemorrhagic enteritis virus specific antibodies in turkey sera. Validation of the test was performed by comparison with a commercially available ELISA test. Serological reactivity was assessed on a panel of 126 turkey sera by a newly developed ELISA test. Serum samples were taken from turkey farms with the history of hemorrhagic enteritis virus infection, from farms with animals free of infection, and from turkey farms following vaccination. Both ELISA kits gave identical results (100 %) with the tested sera. ELISA based on the recombinant hexon protein thus proved useful and cheaper for detection of antibodies in turkey flocks infected with the hemorrhagic enteritis virus.

• MeSH
• Adenoviridae klasifikace   genetika   imunologie   MeSH
• antigeny virové genetika   imunologie   MeSH
• ELISA MeSH
• exprese genu * MeSH
• krocani MeSH
• protilátky virové krev   imunologie   MeSH
• rekombinantní proteiny genetika   imunologie   izolace a purifikace   MeSH
• specificita protilátek imunologie   MeSH
• virové plášťové proteiny genetika   imunologie   izolace a purifikace   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny virové MeSH
• hexon capsid protein, Adenovirus MeSH Prohlížeč
• protilátky virové MeSH
• rekombinantní proteiny MeSH
• virové plášťové proteiny MeSH

Natural recombinant Plum pox virus (PPV) isolates were detected in Albania, Bulgaria, Czech Republic, Germany, Hungary and Slovakia. Despite different geographical origins and dates of isolation, all the recombinant isolates were closely related at the molecular level and shared the same recombination breakpoint as well as a typical signature in their N-terminal coat protein sequence, suggesting a common origin. Biological assays with four recombinant isolates demonstrated their capacity to be aphid-transmitted to various Prunus hosts. One of these isolates had a threonine-to-isoleucine mutation in the conserved PTK motif of its HC-Pro and showed a drastically decreased, although not abolished, aphid transmissibility. The complete genome sequence of one of the recombinant isolates, BOR-3, was determined, as well as some partial sequences in the HC-Pro and P3 genes for additional natural recombinant isolates. Analysis of the phylogenetic relationships between the recombinant isolates and other sequenced PPV isolates confirmed that the recombinant isolates form a phylogenetically homogeneous lineage. In addition, this analysis revealed an ancient recombination event between the PPV-D and M subgroups, with a recombination breakpoint located in the P3 gene. Taken together, these results indicate that recombinant isolates represent an evolutionarily successful, homogeneous group of isolates with a common history and unique founding recombination event. The name PPV-Rec is proposed for this coherent ensemble of isolates.

• MeSH
• fylogeneze MeSH
• genom virový * MeSH
• hmyz - vektory MeSH
• listy rostlin MeSH
• molekulární sekvence - údaje MeSH
• mšice MeSH
• nemoci rostlin virologie   MeSH
• rekombinace genetická MeSH
• sekvenční homologie MeSH
• slivoň virologie   MeSH
• tabák MeSH
• virové geny MeSH
• virové plášťové proteiny genetika   MeSH
• virulence genetika   MeSH
• virus šarky švestky klasifikace   genetika   patogenita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Evropa MeSH
• Názvy látek
• virové plášťové proteiny MeSH