OBJECTIVE: The purpose of the present study was to investigate if participants in NANOK study (National Normative Study of Cognitive Determinants of Healthy Ageing) who show no cognitive decline throughout five years (successful healthy agers; SHA) will show less age-related differences in instrumental activities of daily living (IADL) based on Functional Activities Questionnaire in comparison to participants who show subtle cognitive decline (Decliners) over time. METHOD: We used two different classifications of SHA: Rogalski (N = 25 SHA and N = 15 Decliners) based on cross-sectional neuropsychology measures and linear mixed model (LMEM; 20 SHA and 20 Decliners) based on the Montreal Cognitive Assessment longitudinal 5-years follow-up. Whole-brain T1- and T2-weighted images were corrected for distortions and segmented using Freesurfer. Whole-brain volumetry was performed using FSL's voxel-based morphometry tool. RESULTS: The cognitive decline after four years follow-up but not age predicts subtle impairment in IADL in healthy ageing participants. We found brain volumetric differences between SHA and Decliners based on Rogalski but not LMEM classification especially in bilateral insular cortices and ventrolateral frontal cortex. The logistic regression model achieved an accuracy of 75% for the Rogalski in comparison to 67.5% for the LMEM classification. CONCLUSIONS: Slight restrictions in IADL seem to be a useful tool for screening healthy ageing participants at risk of developing subtle cognitive decline over a period of five years and the cross-sectional Rogalski criteria based on standardized neuropsychological measures were superior for tapping age-related brain changes to longitudinal LMEM classification based on screening (Montreal Cognitive Assessment).

• Klíčová slova
• Activities of daily living, MRI, ageing, brain maintenance, longitudinal study, successful memory ageing,
• MeSH
• činnosti denního života MeSH
• kognice MeSH
• kognitivní dysfunkce * diagnóza   MeSH
• lidé MeSH
• neuropsychologické testy MeSH
• průřezové studie MeSH
• zdravé stárnutí * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The main study objective was to investigate the effect of interactive television-based cognitive training on cognitive performance of 119 healthy older adults, aged 60-87 years. Participants were randomly allocated to a cognitive training group or to an active control group in a single-blind controlled two-group design. Before and after training interactive television cognitive performance was assessed on well validated tests of fluid, higher-order ability, and system usability was evaluated. The participants in the cognitive training group completed a television-based cognitive training programme, while the participants in the active control group completed a TV-based programme of personally benefiting activities. Significant improvements were observed in well validated working memory and executive function tasks in the cognitive training but not in the control group. None of the groups showed statistically significant improvement in life satisfaction score. Participants' reports of "adequate" to "high" system usability testify to the successful development and implementation of the interactive television-based system and compliant cognitive training contents. The study demonstrates that cognitive training delivered by means of an interactive television system can generate genuine cognitive benefits in users and these are measurable using well-validated cognitive tests. Thus, older adults who cannot use or afford a computer can easily use digital interactive television to benefit from advanced software applications designed to train cognition.

• MeSH
• exekutivní funkce * MeSH
• jednoduchá slepá metoda MeSH
• kognice * MeSH
• krátkodobá paměť * MeSH
• kvalita života MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• televize MeSH
• test cesty MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

A small proportion of chronic myeloid leukemia patients treated with interferon-α (IFN-α) monotherapy are able to discontinue the treatment without disease relapse although residual leukemia cells are present. Recently, we showed that these patients have increased amount of NK-cells and a distinct blood cytokine profile. We now aimed to study the function of NK- and T-cells in order to understand the role of the immune system in maintaining the treatment response after IFN-α discontinuation. The study included 13 patients: 5 patients were still treated with IFN-α monotherapy (IFN-ON, median treatment time 163 months) and 8 had stopped the treatment successfully (IFN-OFF, median time without therapy 42 months). Detailed immunophenotype and cytokine production of NK- and T-cells was analyzed with flow cytometry. In addition, the cytotoxicity of NK-cells was studied using K562 as target cells and both the degranulation and direct killing was measured. Compared to healthy controls, IFN-OFF patients had increased proportion of CD4(+) effector memory (CCR7(-)CD45RA(-); median 23% vs. healthy 16%, p = 0.009) and CD8(+) central memory T-cells (CCR7(+)CD45RA(-); median 26% vs. healthy 14%, p = 0.004). Further, upon stimulation the IFN-γ/TNF-α cytokine secretion by CD4(+) T-cells was significantly enhanced in IFN-OFF patients (median 13.7% vs. healthy 7.8%, p = 0.01), and CD4+ effector and central memory cells were the main cytokine producers. No similar increase was observed in IFN-ON group (6.5%). In addition, the proportion of NK-cells was significantly increased in IFN-OFF patients (median IFN-OFF 24%, healthy 13%, p = 0.04), but their direct killing of K562 cells was impaired. The cytotoxicity of NK-cells was also diminished in IFN-ON patients. To conclude, in addition to elevated NK-cell count, IFN-OFF patients have increased amount of memory T-cells, which are able to induce strong cytokine response upon stimulation. This activity may contribute to the maintenance of prolonged remission after successful IFN-α discontinuation.

• MeSH
• buňky K562 MeSH
• buňky NK imunologie   metabolismus   patologie   MeSH
• chronická myeloidní leukemie krev   farmakoterapie   imunologie   patologie   MeSH
• dospělí MeSH
• imunologická paměť účinky léků   MeSH
• imunologické faktory aplikace a dávkování   MeSH
• interferon alfa aplikace a dávkování   MeSH
• lidé středního věku MeSH
• lidé MeSH
• počet CD4 lymfocytů MeSH
• Th1 buňky imunologie   metabolismus   patologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• multicentrická studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• imunologické faktory MeSH
• interferon alfa MeSH

The method of RIA for antibodies was employed with success in VZ virus serology. The method is suitable for testing VZ antibodies in the course of varicella or herpes zoster disease as well as for determining anamnestic titres. Its advantages are stability of antigen, objective reading of results and applicability to testing large serum sets.

• MeSH
• dítě MeSH
• dospělí MeSH
• herpes zoster imunologie   MeSH
• imunologická paměť MeSH
• komplement fixační testy MeSH
• lidé středního věku MeSH
• lidé MeSH
• plané neštovice imunologie   MeSH
• předškolní dítě MeSH
• protilátky virové analýza   MeSH
• radioimunoanalýza MeSH
• senioři MeSH
• virus varicella zoster imunologie   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• předškolní dítě MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protilátky virové MeSH

The paper deals with a possibility of foreign language learning (FLL) intervention in older adults as a psychosocial rehabilitation method to improve the quality of life (QoL) in this age segment, i.e. the people who are over 55 years. FLL has been researched as a successful tool to maintain or even enhance cognitive functions in older age along with other intentional activities, such as engagement in any physical activity. FLL cannot dramatically improve memory and cognitive deterioration of older adults, however, it can improve QoL by increasing subjective happiness that is connected to improved wellbeing. The research was conducted in two groups of seniors who are engaged in FLL, specifically in the Czech Republic and Poland. The major premise of the research was based on the positive psychology concept, i.e., the subjective happiness leads to improved levels of QoL. Both groups consisted of about a hundred respondents whose opinions were researched by an online questionnaire. The major focus of this questionnaire was to evaluate the level of subjective happiness and then compare the results obtained from the respondents from these two geographically different regions. The findings clearly showed that those who had engaged in FLL had reached high levels of subjective happiness, therefore, their subjective wellbeing could be improved. These results might be important for psychosocial rehabilitation practices because they can create a framework for further non-pharmacological intervention to maintain healthy aging. FLL can thus be a very efficient tool for any psychosocial rehabilitation in older healthy adults who do not suffer from any cognitive pathological development but who are just experiencing negative side effects of natural aging process. The research into this topic is very scarce, and therefore, this paper could be an inspiration for further and larger-scale research.

• Klíčová slova
• Aging, Cognitive decline, FLL, Foreign language learning, Healthy aging, L2 acquisition, Psycholinguistics, Seniors, Successful aging, Wellbeing,
• MeSH
• kognice MeSH
• kvalita života * MeSH
• lidé MeSH
• psychiatrická rehabilitace * MeSH
• senioři MeSH
• učení MeSH
• vývoj řeči MeSH
• Check Tag
• lidé MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH

Cognitive flexibility is a major requirement for successful behavior. nNeural oscillations in the alpha frequency band were repeatedly associated with cognitive flexibility in task-switching paradigms. Alpha frequencies are modulated by working memory load and are used to process information during task switching, however we do not know how this oscillatory network communication is modulated. In order to understand the mechanisms that drive cognitive flexibility, ERPs, oscillatory power and how the communication within these networks is organized are of importance. The EEG data show that during phases reflecting preparatory processes to pre-activate task sets, alpha oscillatory power but not the small world properties of the alpha network architecture was modulated. During the switching only the N2 ERP component showed clear modulations. After the response, alpha oscillatory power reinstates and therefore seems to be important to deactivate or maintain the previous task set. For these reactive control processes the network architecture in terms of small-world properties is modulated. Effects of memory load on small-world aspects were seen in repetition trials, where small-world properties were higher when memory processes were relevant. These results suggest that the alpha oscillatory network becomes more small-world-like when reactive control processes during task switching are less complex.

• MeSH
• dospělí MeSH
• elektroencefalografie * MeSH
• evokované potenciály MeSH
• kognice fyziologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mapování mozku MeSH
• mladiství MeSH
• mladý dospělý MeSH
• paměť fyziologie   MeSH
• reakční čas MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Research into face processing consistently shows an outgroup disadvantage in areas such as recognition memory and emotional identification. Potential ingroup advantage with respect to inferences regarding personality and behavioural outcomes, on the other hand, has not yet been studied. In the present study, we used the faces of male professional mixed martial arts (MMA) fighters of apparent African, European, or mixed-race origin as targets and males from four distant populations that vary in ethnic composition as perceivers. We compared the perceivers' inferences about targets' aggressiveness with the fighters' actual performance in professional MMA championships. Surprisingly, across three distant populations used in the study (Cameroon, Czech Republic, and Turkey), perceivers' inferences based on face rating were more congruent with real-world performance for targets belonging to an apparent racial outgroup (as opposed to ingroup). In an ethnically mixed population (Brazil), perceivers showed the lowest congruence for apparently mixed-race targets. It thus seems that the outgroup disadvantage observed in other face processing domains does not carry over to inferences about aggressive behavioural outcomes. In fact, it seems that this relationship is, if anything, reversed.

• MeSH
• agrese fyziologie   MeSH
• dospělí MeSH
• emoce fyziologie   MeSH
• interpersonální vztahy MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• rozpoznávání obrazu fyziologie   MeSH
• sociální percepce * MeSH
• srovnání kultur * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Perspective taking is the ability to imagine what a scene looks like from a different viewpoint, which has been reported to be impaired in Alzheimer's disease (AD). This study compared overhead and first-person view perspective taking abilities in patients with mild cognitive impairment (MCI) and AD. A newly developed Arena Perspective Taking Task (APTT), using an environment of a circular arena, was used to compare 23 AD patients and 38 amnestic MCI patients with 18 healthy controls. The results were contrasted with a published perspective taking test (Standardized Road-Map Test of Direction Sense, RMTDS). The AD group was impaired in both overhead and first-person view APTT versions, but the impairment in the overhead view version applied specifically to women. Patients with aMCI were impaired in the first-person view but not in the overhead view version. Substantial sexual differences were found in the overhead but not in the first-person view APTT version. The RMTDS resembled both APTT versions: patients with aMCI were impaired in this test and also women in both patient groups were less accurate than men. Using the receiver operating characteristic analysis, the highest predictive power for MCI and AD patients diagnosis versus controls was observed for their success rate in the first-person view version. The results suggest distinction between overhead and first-person view perspective taking in the impairment of aMCI patients and the sex differences. The first-person view perspective taking is a potentially important candidate psychological marker for AD.

• Klíčová slova
• Alzheimer's disease, Mild cognitive impairment, Perspective taking task, Spatial transformation, Standardized Road-Map Test of Direction Sense,
• MeSH
• Alzheimerova nemoc psychologie   MeSH
• amnézie psychologie   MeSH
• kognice * MeSH
• kognitivní dysfunkce psychologie   MeSH
• lidé MeSH
• neuropsychologické testy MeSH
• plnění a analýza úkolů MeSH
• průřezové studie MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• stárnutí psychologie   MeSH
• studie případů a kontrol MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Helicobacter pylori (Hp) contributes to the development of gastric and extra-gastric diseases such as autoimmune thyroiditis (AT), and causes persistent life-long infection despite local and systemic immune response. We determined the specific cellular immune response to Hp antigens and PWM (control mitogen) in two groups of Hp infected patients--group A (n = 21), involving patients with autoimmune thyroiditis and group B (n = 13) of patients without AT--using modified lymphocyte transformation test before and after eradication therapy in comparison with healthy controls (group C, n = 15). Immune reactivity to the majority of Hp antigens (aHp, hHp, HpAg, CagA) was significantly lower in group B before eradication therapy in comparison with healthy Hp negative controls. A significant increase in immune reactivity was observed in group B to certain Hp antigens after successful eradication. The same levels (but insignificant) of immune reactivity were shown in group A. Our results indicate that Hp can cause the inhibition of the specific cellular immune response in Hp infected patients with or without autoimmune diseases such as AT, which can be abrogated by successful eradication of Hp. Lymphocyte transformation test appears to be a good tool for detection of immune memory cellular response in patients with Hp infection.

• MeSH
• antigeny bakteriální imunologie   MeSH
• autoimunitní tyreoiditida komplikace   imunologie   MeSH
• dospělí MeSH
• Helicobacter pylori imunologie   MeSH
• imunologická tolerance MeSH
• infekce vyvolané Helicobacter pylori komplikace   imunologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lymfocyty imunologie   MeSH
• proliferace buněk MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny bakteriální MeSH

OBJECT: Idiopathic normal pressure hydrocephalus (iNPH) is the only variant of dementia disorders possibly treatable by neurosurgical intervention. iNPH is a neurodegenerative condition clinically characterized by gait ataxia, urinary incontinence, and memory disturbance. We present one of the largest single-center studies, which was designed to prove efficacy of our low-pressure setting of gravitational valve at all three symptoms of iNPH and to find statistically significant cut-off time for best clinical improvement according to the duration of symptoms. METHODS: Sixty-one consecutive patients (mean age 74.9 ± 5.3) with iNPH were prospectively observed from the time of surgery with minimal 6 months follow-up. All patients underwent implantation of the same type of gravitational valve with the same setting-pro GAV with low opening pressure at 5 cm H2O-and were operated by the same team of 2 neurosurgeons. We statistically evaluated gait disturbance, psychological changes, and incontinence preoperatively and at 6 months after surgery and timing of the surgery according to the duration of symptoms and to the age. RESULTS: Paired t test showed a statistically significant increase in MMSE, a statistically significant decrease in 10-m walk test and 360 deg. rotation test (p < 0.0001). The correlation among the change of the MMSE, the walk test, and the rotation test, and the age and time of symptoms' duration was verified by Pearson's correlation coefficient. Pearson's correlation coefficient showed a medium strong correlation between the change of MMSE and the time of symptoms (r = - 0.580; p < 0.0001) and between the change of the number of steps and the time of symptoms (r = 0.517, p < 0.0001). There was a statistically significant weak (poor) correlation between the change of the walk test and the time of symptoms (r = 0.351, p = 0.006). All 3 ROC tests confirmed optimal cut-off for the best improvement of symptoms as 9.5 months of the symptom duration. CONCLUSIONS: We proved statistical significant optimal cut-off for the best improvement of the symptoms as 9.5 months of the symptom duration. This study also confirmed successful treatment of iNPH with VP shunting using low pressure setting of gravitational valve with overall improvement in 75% and low over drainage complications in 5% We proved statistically significant increase in MMSE, decrease in 10 m walk test and number of steps test, p < 0.0001.

• Klíčová slova
• Normal pressure hydrocephalus, VP shunt, prospective study,
• MeSH
• lidé středního věku MeSH
• lidé MeSH
• normotenzní hydrocefalus chirurgie   MeSH
• pooperační komplikace epidemiologie   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ventrikuloperitoneální zkrat škodlivé účinky   přístrojové vybavení   metody   MeSH
• výsledek terapie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH