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Two approaches to biological decontamination of groundwater and soil polluted by aromatics-characterization of microbial populations
Demnerová K, Mackova M, Spevákova V, Beranova K, Kochánková L, Lovecká P, Ryslavá E, Macek T.
Language English Country Spain
NLK
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from 1998 to 2017
- MeSH
- Actinobacteria genetics classification metabolism MeSH
- Hydrocarbons, Aromatic metabolism MeSH
- Biodegradation, Environmental MeSH
- Water Pollutants, Chemical metabolism MeSH
- Decontamination methods MeSH
- Ecosystem MeSH
- Financing, Organized MeSH
- Plant Roots microbiology MeSH
- Soil Pollutants metabolism MeSH
- Proteobacteria genetics classification metabolism MeSH
- Soil Microbiology MeSH
- Plants microbiology MeSH
As part of the EU project MULTIBARRIERS, six new endogenous aerobic bacterial isolates able to grow in the presence of BTmX (benzene, toluene, m-xylene) were characterized with respect to their growth specificities. Preliminary analysis included restriction fragment length polymorphism profiles and 16S rDNA sequencing. The diversity of these strains was confirmed by denaturing gradient gel electrophoresis. Additional aerobic bacterial strains were isolated from the rhizospheres of plants grown in polychlorinated biphenyl (PCB)-contaminated soils. Pot experiments were designed to show the beneficial effect of plants on the bacterial degradation of PCBs. The effect of PCB removal from soil was evaluated and bacteria isolated from three different plant species were examined for the presence of the bph operon.
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- $a Dpt. of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Inst. of Chemical Technology Prague, Czech Republic. Katerina.Demnerova@vscht.cz
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- $a As part of the EU project MULTIBARRIERS, six new endogenous aerobic bacterial isolates able to grow in the presence of BTmX (benzene, toluene, m-xylene) were characterized with respect to their growth specificities. Preliminary analysis included restriction fragment length polymorphism profiles and 16S rDNA sequencing. The diversity of these strains was confirmed by denaturing gradient gel electrophoresis. Additional aerobic bacterial strains were isolated from the rhizospheres of plants grown in polychlorinated biphenyl (PCB)-contaminated soils. Pot experiments were designed to show the beneficial effect of plants on the bacterial degradation of PCBs. The effect of PCB removal from soil was evaluated and bacteria isolated from three different plant species were examined for the presence of the bph operon.
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