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Real-time quantitative PCR detection of Mycobacterium avium subspecies in meat products
B. Klanicova, I. Slana, H. Vondruskova, M. Kaevska, I. Pavlik
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Freely Accessible Science Journals
from 1977 to 5 years ago
ProQuest Central
from 2009-01-01 to 2022-12-31
Health & Medicine (ProQuest)
from 2009-01-01 to 2022-12-31
Public Health Database (ProQuest)
from 2009-01-01 to 2022-12-31
ROAD: Directory of Open Access Scholarly Resources
- MeSH
- DNA, Bacterial analysis MeSH
- Species Specificity MeSH
- Food Contamination analysis MeSH
- Humans MeSH
- Meat Products microbiology MeSH
- Mycobacterium avium classification isolation & purification MeSH
- Polymerase Chain Reaction methods MeSH
- Prevalence MeSH
- Consumer Product Safety MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The aim of this work was to examine various purchased meat products and to find out if any traces of Mycobacterium avium subsp. avium, M. avium subsp. hominissuis, and M. avium subsp. paratuberculosis could be detected in these samples. Analysis of the meat products (raw, cooked, and fermented) was performed using a real-time quantitative PCR (qPCR) method for the detection of specific insertion sequences: duplex qPCR for the detection of IS900 specific for M. avium subsp. paratuberculosis, and triplex qPCR for the detection of IS901 specific for Mycobacterium avium subsp. avium and IS 1245 specific for M. avium subsp. hominissuis. Of the 77 analyzed meat samples, 17 (22%) were found to contain M. avium subsp. paratuberculosis DNA, 4 (5%) samples contained Mycobacterium avium subsp. avium DNA, and in 12 (16%) samples M. avium subsp. hominissuis DNA was detected. The concentration of M. avium subsp. paratuberculosis and M. avium subsp. hominissuis DNA in some meat products exceeded 10(4) genomes per g. Culture examination of these mycobacterial subspecies was negative. By analyzing a range of meat products, we have provided evidence to support the hypothesis that M. avium is present in everyday commodities sold to the general public.
References provided by Crossref.org
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