• Je něco špatně v tomto záznamu ?

Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples

G. Papadakis, P. Murasova, A. Hamiot, K. Tsougeni, G. Kaprou, M. Eck, D. Rabus, Z. Bilkova, B. Dupuy, G. Jobst, A. Tserepi, E. Gogolides, E. Gizeli,

. 2018 ; 111 (-) : 52-58. [pub] 20180328

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu hodnotící studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033173

The fast and efficient detection of foodborne pathogens is a societal priority, given the large number of food-poisoning outbreaks, and a scientific and technological challenge, given the need to detect as little as 1 viable cell in 25 gr of food. Here, we present the first approach that achieves the above goal, thanks to the use of a micro/nano-technology and the detection capability of acoustic wave sensors. Starting from 1 Salmonella cell in 25 ml of milk, we employ immuno-magnetic beads to capture cells after only 3 h of pre-enrichment and subsequently demonstrate efficient DNA amplification using the Loop Mediated Isothermal Amplification method (LAMP) and acoustic detection in an integrated platform, within an additional ½ h. The demonstrated 4 h sample-to-analysis time comes as a huge improvement to the current need of few days to obtain the same result. In addition, the work presents the first reported Lab-on-Chip platform that comprises an acoustic device as the sensing element, exhibiting impressive analytical features, namely, an acoustic limit of detection of 2 cells/μl or 3 aM of the DNA target and ability to detect in a label-free manner dsDNA amplicons in impure samples. The use of food samples together with the incorporation of the necessary pre-enrichment step and ability for multiple analysis with an internal control, make the proposed methodology highly relevant to real-world applications. Moreover, the work suggests that acoustic wave devices can be used as an attractive alternative to electrochemical sensors in integrated platforms for applications in food safety and the point-of-care diagnostics.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18033173
003      
CZ-PrNML
005      
20181022130148.0
007      
ta
008      
181008s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.bios.2018.03.056 $2 doi
035    __
$a (PubMed)29635118
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Papadakis, George $u Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str., Heraklion 70013, Greece. Electronic address: gpapadak@imbb.forth.gr.
245    10
$a Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples / $c G. Papadakis, P. Murasova, A. Hamiot, K. Tsougeni, G. Kaprou, M. Eck, D. Rabus, Z. Bilkova, B. Dupuy, G. Jobst, A. Tserepi, E. Gogolides, E. Gizeli,
520    9_
$a The fast and efficient detection of foodborne pathogens is a societal priority, given the large number of food-poisoning outbreaks, and a scientific and technological challenge, given the need to detect as little as 1 viable cell in 25 gr of food. Here, we present the first approach that achieves the above goal, thanks to the use of a micro/nano-technology and the detection capability of acoustic wave sensors. Starting from 1 Salmonella cell in 25 ml of milk, we employ immuno-magnetic beads to capture cells after only 3 h of pre-enrichment and subsequently demonstrate efficient DNA amplification using the Loop Mediated Isothermal Amplification method (LAMP) and acoustic detection in an integrated platform, within an additional ½ h. The demonstrated 4 h sample-to-analysis time comes as a huge improvement to the current need of few days to obtain the same result. In addition, the work presents the first reported Lab-on-Chip platform that comprises an acoustic device as the sensing element, exhibiting impressive analytical features, namely, an acoustic limit of detection of 2 cells/μl or 3 aM of the DNA target and ability to detect in a label-free manner dsDNA amplicons in impure samples. The use of food samples together with the incorporation of the necessary pre-enrichment step and ability for multiple analysis with an internal control, make the proposed methodology highly relevant to real-world applications. Moreover, the work suggests that acoustic wave devices can be used as an attractive alternative to electrochemical sensors in integrated platforms for applications in food safety and the point-of-care diagnostics.
650    _2
$a akustika $x přístrojové vybavení $7 D000162
650    _2
$a zvířata $7 D000818
650    _2
$a biosenzitivní techniky $x přístrojové vybavení $7 D015374
650    _2
$a DNA bakterií $x analýza $x genetika $7 D004269
650    _2
$a design vybavení $7 D004867
650    _2
$a analýza potravin $x přístrojové vybavení $7 D005504
650    _2
$a kontaminace potravin $x analýza $7 D005506
650    _2
$a potravinářská mikrobiologie $7 D005516
650    _2
$a nemoci přenášené potravou $x mikrobiologie $7 D005517
650    _2
$a lidé $7 D006801
650    _2
$a laboratoř na čipu $7 D056656
650    _2
$a limita detekce $7 D057230
650    _2
$a mléko $x mikrobiologie $7 D008892
650    _2
$a Salmonella $x genetika $x izolace a purifikace $7 D012475
650    _2
$a salmonelóza $x mikrobiologie $7 D012480
650    _2
$a zvuk $7 D013016
655    _2
$a hodnotící studie $7 D023362
655    _2
$a časopisecké články $7 D016428
700    1_
$a Murasova, Pavla $u University of Pardubice, 573 Studentska, Pardubice 532 10, Czech Republic.
700    1_
$a Hamiot, Audrey $u Institut Pasteur, 25-28 Rue du Docteur Roux, Paris Cedex 15 75724, France.
700    1_
$a Tsougeni, Katerina $u Institute of Nanoscience and Nanotechnology, NCSR-Demokritos, Patriarhou Gregoriou & 27 Neapoleos Str., Aghia Paraskevi, Attiki 15341, Greece.
700    1_
$a Kaprou, Georgia $u Department of Biology, Univ. of Crete, Voutes, Heraklion 70013, Greece; Institute of Nanoscience and Nanotechnology, NCSR-Demokritos, Patriarhou Gregoriou & 27 Neapoleos Str., Aghia Paraskevi, Attiki 15341, Greece.
700    1_
$a Eck, Michael $u Jobst Technologies GmbH, Engesserstraße 4b, 79108 Freiburg, Germany.
700    1_
$a Rabus, David $u SENSeOR SAS, Temis Innovation, 18 rue Alain Savary, 25000 Besançon, France.
700    1_
$a Bilkova, Zuzana $u University of Pardubice, 573 Studentska, Pardubice 532 10, Czech Republic.
700    1_
$a Dupuy, Bruno $u Institut Pasteur, 25-28 Rue du Docteur Roux, Paris Cedex 15 75724, France.
700    1_
$a Jobst, Gerhard $u Jobst Technologies GmbH, Engesserstraße 4b, 79108 Freiburg, Germany.
700    1_
$a Tserepi, Angeliki $u Institute of Nanoscience and Nanotechnology, NCSR-Demokritos, Patriarhou Gregoriou & 27 Neapoleos Str., Aghia Paraskevi, Attiki 15341, Greece.
700    1_
$a Gogolides, Evangelos $u Institute of Nanoscience and Nanotechnology, NCSR-Demokritos, Patriarhou Gregoriou & 27 Neapoleos Str., Aghia Paraskevi, Attiki 15341, Greece.
700    1_
$a Gizeli, Electra $u Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str., Heraklion 70013, Greece; Department of Biology, Univ. of Crete, Voutes, Heraklion 70013, Greece. Electronic address: gizeli@imbb.forth.gr.
773    0_
$w MED00006627 $t Biosensors & bioelectronics $x 1873-4235 $g Roč. 111, č. - (2018), s. 52-58
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29635118 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20181008 $b ABA008
991    __
$a 20181022130654 $b ABA008
999    __
$a ok $b bmc $g 1340040 $s 1030167
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 111 $c - $d 52-58 $e 20180328 $i 1873-4235 $m Biosensors & bioelectronics $n Biosens Bioelectron $x MED00006627
LZP    __
$a Pubmed-20181008

Najít záznam

Citační ukazatele

Možnosti archivace