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Genomic and Functional Analysis of Emerging Virulent and Multidrug-Resistant Escherichia coli Lineage Sequence Type 648
K. Schaufler, T. Semmler, LH. Wieler, DJ. Trott, J. Pitout, G. Peirano, J. Bonnedahl, M. Dolejska, I. Literak, S. Fuchs, N. Ahmed, M. Grobbel, C. Torres, A. McNally, D. Pickard, C. Ewers, NJ. Croucher, J. Corander, S. Guenther,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
Wellcome Trust - United Kingdom
NLK
Free Medical Journals
od 1972 do Před 6 měsíci
Freely Accessible Science Journals
od 1995 do Před 6 měsíci
PubMed Central
od 1972 do Před 6 měsíci
Europe PubMed Central
od 1972 do Před 6 měsíci
Open Access Digital Library
od 1972-01-01
Open Access Digital Library
od 1972-01-01
PubMed
30885899
DOI
10.1128/aac.00243-19
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriemie farmakoterapie mikrobiologie MeSH
- beta-laktamasy genetika MeSH
- biofilmy účinky léků MeSH
- Escherichia coli účinky léků genetika MeSH
- faktory virulence genetika MeSH
- genomika metody MeSH
- infekce močového ústrojí farmakoterapie mikrobiologie MeSH
- infekce vyvolané Escherichia coli farmakoterapie mikrobiologie MeSH
- kur domácí mikrobiologie MeSH
- lidé MeSH
- mnohočetná bakteriální léková rezistence genetika MeSH
- multilokusová sekvenční typizace metody MeSH
- plazmidy genetika MeSH
- sekvenování celého genomu metody MeSH
- virulence genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The pathogenic extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global ST648 collection from various hosts (87 whole-genome sequences) combining core and accessory genomics with functional analyses and in vivo experiments suggests that ST648 is a nascent and generalist lineage, lacking clear phylogeographic and host association signals. By including large numbers of ST131 (n = 107) and ST10 (n = 96) strains for comparative genomics and phenotypic analysis, we demonstrate that the combination of multidrug resistance and high-level virulence are the hallmarks of ST648, similar to international high-risk clonal lineage ST131. Specifically, our in silico, in vitro, and in vivo results demonstrate that ST648 is well equipped with biofilm-associated features, while ST131 shows sophisticated signatures indicative of adaption to urinary tract infection, potentially conveying individual ecological niche adaptation. In addition, we used a recently developed NFDS (negative frequency-dependent selection) population model suggesting that ST648 will increase significantly in frequency as a cause of bacteremia within the next few years. Also, ESBL plasmids impacting biofilm formation aided in shaping and maintaining ST648 strains to successfully emerge worldwide across different ecologies. Our study contributes to understanding what factors drive the evolution and spread of emerging international high-risk clonal lineages.
Faculty of Medicine School of Public Health Imperial College London United Kingdom
Institute of Microbiology and Infection University of Birmingham Birmingham United Kingdom
International Center for Diarrheal Disease Research Bangladesh Dhaka Bangladesh
NG 1 Microbial Genomics Robert Koch Institute Berlin Germany
Robert Koch Institute Berlin Germany
The Wellcome Trust Sanger Institute Cambridge United Kingdom
Universidad de La Rioja Area de Bioquímica y Biología Molecular Logroño Spain
Citace poskytuje Crossref.org
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- $a Schaufler, Katharina $u Pharmaceutical Microbiology, Institute of Pharmacy, University of Greifswald, Greifswald, Germany. Institute of Microbiology and Epizootics, Free University Berlin, Berlin, Germany.
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- $a The pathogenic extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global ST648 collection from various hosts (87 whole-genome sequences) combining core and accessory genomics with functional analyses and in vivo experiments suggests that ST648 is a nascent and generalist lineage, lacking clear phylogeographic and host association signals. By including large numbers of ST131 (n = 107) and ST10 (n = 96) strains for comparative genomics and phenotypic analysis, we demonstrate that the combination of multidrug resistance and high-level virulence are the hallmarks of ST648, similar to international high-risk clonal lineage ST131. Specifically, our in silico, in vitro, and in vivo results demonstrate that ST648 is well equipped with biofilm-associated features, while ST131 shows sophisticated signatures indicative of adaption to urinary tract infection, potentially conveying individual ecological niche adaptation. In addition, we used a recently developed NFDS (negative frequency-dependent selection) population model suggesting that ST648 will increase significantly in frequency as a cause of bacteremia within the next few years. Also, ESBL plasmids impacting biofilm formation aided in shaping and maintaining ST648 strains to successfully emerge worldwide across different ecologies. Our study contributes to understanding what factors drive the evolution and spread of emerging international high-risk clonal lineages.
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