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Genomic and Functional Analysis of Emerging Virulent and Multidrug-Resistant Escherichia coli Lineage Sequence Type 648
K. Schaufler, T. Semmler, LH. Wieler, DJ. Trott, J. Pitout, G. Peirano, J. Bonnedahl, M. Dolejska, I. Literak, S. Fuchs, N. Ahmed, M. Grobbel, C. Torres, A. McNally, D. Pickard, C. Ewers, NJ. Croucher, J. Corander, S. Guenther,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Wellcome Trust - United Kingdom
NLK
Free Medical Journals
from 1972 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1972 to 1 year ago
Europe PubMed Central
from 1972 to 6 months ago
Open Access Digital Library
from 1972-01-01
Open Access Digital Library
from 1972-01-01
PubMed
30885899
DOI
10.1128/aac.00243-19
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacteremia drug therapy microbiology MeSH
- beta-Lactamases genetics MeSH
- Biofilms drug effects MeSH
- Escherichia coli drug effects genetics MeSH
- Virulence Factors genetics MeSH
- Genomics methods MeSH
- Urinary Tract Infections drug therapy microbiology MeSH
- Escherichia coli Infections drug therapy microbiology MeSH
- Chickens microbiology MeSH
- Humans MeSH
- Drug Resistance, Multiple, Bacterial genetics MeSH
- Multilocus Sequence Typing methods MeSH
- Plasmids genetics MeSH
- Whole Genome Sequencing methods MeSH
- Virulence genetics MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The pathogenic extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global ST648 collection from various hosts (87 whole-genome sequences) combining core and accessory genomics with functional analyses and in vivo experiments suggests that ST648 is a nascent and generalist lineage, lacking clear phylogeographic and host association signals. By including large numbers of ST131 (n = 107) and ST10 (n = 96) strains for comparative genomics and phenotypic analysis, we demonstrate that the combination of multidrug resistance and high-level virulence are the hallmarks of ST648, similar to international high-risk clonal lineage ST131. Specifically, our in silico, in vitro, and in vivo results demonstrate that ST648 is well equipped with biofilm-associated features, while ST131 shows sophisticated signatures indicative of adaption to urinary tract infection, potentially conveying individual ecological niche adaptation. In addition, we used a recently developed NFDS (negative frequency-dependent selection) population model suggesting that ST648 will increase significantly in frequency as a cause of bacteremia within the next few years. Also, ESBL plasmids impacting biofilm formation aided in shaping and maintaining ST648 strains to successfully emerge worldwide across different ecologies. Our study contributes to understanding what factors drive the evolution and spread of emerging international high-risk clonal lineages.
Faculty of Medicine School of Public Health Imperial College London United Kingdom
Institute of Microbiology and Infection University of Birmingham Birmingham United Kingdom
International Center for Diarrheal Disease Research Bangladesh Dhaka Bangladesh
NG 1 Microbial Genomics Robert Koch Institute Berlin Germany
Robert Koch Institute Berlin Germany
The Wellcome Trust Sanger Institute Cambridge United Kingdom
Universidad de La Rioja Area de Bioquímica y Biología Molecular Logroño Spain
References provided by Crossref.org
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