Modulation of cell surface EGF receptor and HLA expression on glioma cell lines induced with cytokines and differentiation promoters
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
7895857
Knihovny.cz E-resources
- MeSH
- Antigens, Surface drug effects metabolism MeSH
- Cell Differentiation drug effects MeSH
- Cytokines metabolism pharmacology MeSH
- ErbB Receptors drug effects physiology MeSH
- Glioma chemistry immunology MeSH
- HLA Antigens drug effects physiology MeSH
- Interferon-gamma metabolism pharmacology MeSH
- Interleukin-1 metabolism pharmacology MeSH
- Humans MeSH
- Tumor Cells, Cultured drug effects MeSH
- Tetradecanoylphorbol Acetate pharmacology MeSH
- Tumor Necrosis Factor-alpha metabolism pharmacology MeSH
- Tretinoin pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Surface MeSH
- Cytokines MeSH
- ErbB Receptors MeSH
- HLA Antigens MeSH
- Interferon-gamma MeSH
- Interleukin-1 MeSH
- Tetradecanoylphorbol Acetate MeSH
- Tumor Necrosis Factor-alpha MeSH
- Tretinoin MeSH
Alterations of cell surface expression of HLA (class I, class II DR, DP and DQ) and EGF-receptor on two malignant glioma cell lines (U-343MG and U-563MG) induced with cytokines (IFN-gamma, TNF-alpha, IL-1 alpha) and differentiation promoters (all-trans retinoic acid, phorbol ester TPA) were analyzed with the aid of flow cytometry. IFN-gamma induced a 10-15fold increase of HLA class I. TNF-alpha alone induced a two- to fivefold increase of HLA class I cell surface density and increased the IFN-gamma induced upregulation of HLA class I to approximately 20-24 times the antigen density of uninduced cells. TNF-alpha was able to increase HLA class II DR and DP cell surface expression on glioma lines, but it enhanced only the IFN-gamma-induced HLA class II DR upregulation. All-trans retinoic acid and TPA regulated in the opposite way the EGF-receptor cell surface expression on U-563MG cells.
