Effect of hydrogen peroxide on sugar transport in Schizosaccharomyces pombe. Absence of membrane lipid peroxidation
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
8375778
DOI
10.1007/bf02891695
Knihovny.cz E-resources
- MeSH
- Biological Transport, Active drug effects MeSH
- Antimycin A pharmacology MeSH
- Arabinose metabolism MeSH
- Deoxyglucose analogs & derivatives metabolism MeSH
- Fungal Proteins metabolism MeSH
- Carbonyl Cyanide m-Chlorophenyl Hydrazone pharmacology MeSH
- Catalase metabolism MeSH
- Membrane Lipids metabolism MeSH
- Membrane Proteins metabolism MeSH
- Carbohydrate Metabolism * MeSH
- Hydrogen Peroxide pharmacology MeSH
- Lipid Peroxidation * MeSH
- Proton-Translocating ATPases metabolism MeSH
- Schizosaccharomyces drug effects metabolism MeSH
- Oxygen Consumption MeSH
- Carrier Proteins metabolism MeSH
- Xylose metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- 6-deoxyglucose MeSH Browser
- Antimycin A MeSH
- Arabinose MeSH
- Deoxyglucose MeSH
- Fungal Proteins MeSH
- Carbonyl Cyanide m-Chlorophenyl Hydrazone MeSH
- Catalase MeSH
- Membrane Lipids MeSH
- Membrane Proteins MeSH
- Hydrogen Peroxide MeSH
- Proton-Translocating ATPases MeSH
- Carrier Proteins MeSH
- Xylose MeSH
Stationary unaerated cells of S. pombe containing endogenous substrates but not energized by any exogenous ones take up 2-deoxy-D-glucose, 6-deoxy-D-glucose, D-xylose and D-arabinose actively over diffusion equilibrium. The active uptake is inhibited by 20-100 mmol/L H2O2 which causes an increase in KT but has no effect on Jmax. This "competitive inhibition" indicates that H2O2 affects directly the sugar binding sites of the transporters. The ATP-binding site of the plasma membrane H(+)-ATPase is also affected by 100 mmol/L H2O2; the KT decreases 7-fold, Jmax about 2.5-fold. These effects are not likely to be mediated by membrane lipid peroxidation which appears to be lacking in S. pombe, and this lack may be one of the reasons for the high resistance of this yeast to H2O2. Because of this S. pombe represents a suitable system for studying direct effects of oxidants on membrane proteins.
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