Potato virus A detection by reverse transcription-polymerase chain reaction
Language English Country Switzerland Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
9770075
Knihovny.cz E-resources
- MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Polymerase Chain Reaction methods MeSH
- Potyvirus isolation & purification MeSH
- Solanum tuberosum virology MeSH
- Blotting, Southern MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
Simple and reliable procedure for sample preparation and reverse transcription-polymerase chain reaction (RT-PCR) detection of potato virus A (PVA) is described. PVA-specific primers used in the RT-PCR defined a target sequence of 321 bp and did not produce amplification product(s) with potato virus Y.