Status epilepticus causes necrotic damage in the mediodorsal nucleus of the thalamus in immature rats

. 2001 May 15 ; 21 (10) : 3593-9.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid11331388

Status epilepticus (StE) in immature rats causes long-term functional impairment. Whether this is associated with structural alterations remains controversial. The present study was designed to test the hypothesis that StE at an early age results in neuronal loss. StE was induced with lithium-pilocarpine in 12-d-old rats, and the presence of neuronal damage was investigated in the brain from 12 hr up to 1 week later using silver and Fluoro-Jade B staining techniques. Analysis of the sections indicated consistent neuronal damage in the central and lateral segments of the mediodorsal nucleus of the thalamus, which was confirmed using adjacent cresyl violet-stained preparations. The mechanism of thalamic damage (necrosis vs apoptosis) was investigated further using TUNEL, immunohistochemistry for caspase-3 and cytochrome c, and electron microscopy. Activated microglia were detected using OX-42 immunohistochemistry. The presence of silver and Fluoro-Jade B-positive degenerating neurons in the mediodorsal thalamic nucleus was associated with the appearance of OX-42-immunopositive activated microglia but not with the expression of markers of programmed cell death, caspase-3, or cytochrome c. Electron microscopy revealed necrosis of the ultrastructure of damaged neurons, providing further evidence that the mechanism of StE-induced damage in the mediodorsal thalamic nucleus at postnatal day 12 is necrosis rather than apoptosis. Finally, these data together with previously described functions of the medial and lateral segments of the mediodorsal thalamic nucleus suggest that some functions, such as adaptation to novelty, might become compromised after StE early in development.

Zobrazit více v PubMed

Aicardi J, Chevrie JJ. Convulsive status epilepticus in infants and children: a study of 239 cases. Epilepsia. 1970;11:187–197. PubMed

Babb TL, Leite JP, Mathern GW, Pretorius JK. Kainic acid induced hippocampal seizures in rats: comparison of acute and chronic seizures using intrahippocampal versus systemic injection. Ital J Neurol Sci. 1995;16:39–44. PubMed

Cassidy RM, Gale K. Mediodorsal thalamus plays a critical role in the development of limbic motor seizures. J Neurosci. 1998;18:9002–9009. PubMed PMC

Chang D, Baram TZ. Status epilepticus results in reversible neuronal injury in infant rat hippocampus. Dev Brain Res. 1994;77:133–136. PubMed PMC

Chen K, Baram TZ, Soltesz I. Febrile seizures in the developing brain result in persistent modification of neuronal excitability in limbic circuits. Nat Med. 1999;5:888–894. PubMed PMC

Crandall JE, Caviness VS., Jr Axon strata of the cerebral wall in embryonic mice. Brain Res. 1984a;316:185–195. PubMed

Crandall JE, Caviness VS., Jr Thalamocortical connections in newborn mice. J Comp Neurol. 1984b;228:542–556. PubMed

da Silva Fernandes MJ, Dubé C, Boyet S, Marescaux C, Nehlig A. Correlation between hypermetabolism and neural damage during status epilepticus induced by lithium and pilocarpine in immature and adult rats. J Cereb Blood Flow Metab. 1999;19:195–209. PubMed

de Feo MR, Mecarelli O, Palladini G, Ricci GF. Long-term effects of status epilepticus on the acquisition of conditioned avoidance behavior in rats. Epilepsia. 1986;27:476–482. PubMed

Deshpande SS, Smith CD, Filbert MG. Assessment of primary neuronal culture as a model for soman-induced neurotoxicity and effectiveness of memantine as a neuroprotective drug. Arch Toxicol. 1995;69:384–390. PubMed

Dobbing J. Undernutrition and the developing brain. In: Himwich WA, editor. Developmental neurobiology. Charles C Thomas; Springfield: 1970. pp. 241–261.

Dubé C, Boyet S, Marescaux C, Nehlig A. Progressive metabolic changes underlying the chronic reorganization of brain circuits during the silent phase of the lithium-pilocarpine model of epilepsy in the immature and adult rat. Exp Neurol. 2000a;162:146–157. PubMed

Dubé C, Chen K, Eghbal-Ahmadi M, Brunson K, Soltesz I, Baram TZ. Prolonged febrile seizures in the immature rat model enhance hippocampal excitability long term. Ann Neurol. 2000b;47:336–344. PubMed PMC

Gallyas F, Wolff H, Bottcher H, Zaborszky L. A reliable and sensitive method to localize terminal degeneration and lysozymes in the central nervous system. Stain Technol. 1980;55:299–306. PubMed

Groenewegen HJ. Organization of the afferent connections of the mediodorsal thalamic nucleus in the rat, related to the mediodorsal-prefrontal topography. Neuroscience. 1988;24:379–431. PubMed

Harrigan T, Peredery O, Persinger M. Radial maze learning deficits and mediodorsal thalamic damage in context of multifocal seizure-induced brain lesions. Behav Neurosci. 1991;105:482–486. PubMed

Hirsch E, Baram TZ, Snead OC., III Ontogenic study of lithium-pilocarpine-induced status epilepticus in rats. Brain Res. 1992;583:120–126. PubMed

Hort J, Brožek G, Komárek V, Langmeier M, Mareš P. Interstrain differences in cognitive functions in rats in relation to status epilepticus. Behav Brain Res. 2000;112:77–83. PubMed

Hu BR, Liu CL, Ouyang Y, Blomgren K, Siesjö BK. Involvement of caspase-3 in cell death after hypoxia-ischemia declines brain maturation. J Cereb Blood Flow Metab. 2000;20:1294–1300. PubMed

Hussenet F, Boyet S, Nehlig A. Long-term metabolic effects of pentylenetetrazol-induced status epilepticus in the immature brain. Neuroscience. 1995;67:455–461. PubMed

Krettek JE, Price JL. The cortical projections of the mediodorsal nucleus and adjacent thalamic nuclei in the rat. J Comp Neurol. 1977;171:157–192. PubMed

Kubová H, Haugvicová R, Suchomelová L, Mareš P. Does status epilepticus influence motor development of immature rats? Epilepsia. 2000;41 [Suppl 6]:S64–S69. PubMed

Kuroda M, Price JL. Synaptic organization of projections from basal forebrain structures to the mediodorsal thalamic nucleus of the rat. J Comp Neurol. 1991;303:513–533. PubMed

Lund RD, Mustari MJ. Development of the geniculocortical pathway in rats. J Comp Neurol. 1977;173:289–306. PubMed

McCrea RA, Baker R. Anatomical connection of the nucleus prepositus of the cat. J Comp Neurol. 1985;237:377–407. PubMed

Minciacchi D, Granato A. Developmental remodeling of thalamic projections to the frontal cortex in rats. In: Bentivoglio M, Spreafico R, editors. Cellular thalamic mechanisms. Elsevier; Amsterdam: 1988. pp. 502–516.

Morrisett RA, Jope RJ, Snead OC., III Effects of drugs on the initiation and maintenance of status epilepticus induced by administration of pilocarpine to lithium-pretreated rats. Exp Neurol. 1987;97:193–200. PubMed

Nehlig A, Pereira de Vasconcelos A. The model of pentylenetetrazol-induced status epilepticus in the immature rat: short- and long-term effects. Epilepsy Res. 1996;26:93–103. PubMed

Patel S, Millan MH, Meldrum BS. Decrease in excitatory transmission within the lateral habenula and the mediodorsal thalamus protects against limbic seizures in rats. Exp Neurol. 1988;101:63–74. PubMed

Paxinos G, Watson C. The rat brain in stereotaxic coordinates. Academic; New York: 1982. PubMed

Pineau N, Charriaut-Marlangue C, Motte J, Nehlig A. Pentylenetetrazol induced seizures induce cell suffering but not death in the immature brain. Dev Brain Res. 1999;112:139–144. PubMed

Ray JP, Price JL. The organization of projections from the mediodorsal nucleus of the thalamus to orbital and medial prefrontal cortex in macaque monkeys. J Comp Neurol. 1993;337:1–31. PubMed

Ray JP, Russchen FT, Fuller TA, Price JL. Sources of presumptive glutamatergic/aspartatergic afferents to the mediodorsal nucleus of the thalamus in the rat. J Comp Neurol. 1992;320:435–456. PubMed

Sagar HJ, Oxbury JM. Hippocampal neuron loss in temporal lobe epilepsy: correlation with early childhood convulsions. Ann Neurol. 1987;22:334–340. PubMed

Sankar R, Shin DH, Wasterlain CG. Serum neuron-specific enolase is a marker for neuronal damage following status epilepticus in the rat. Epilepsy Res. 1997;28:129–136. PubMed

Sankar R, Shin DH, Liu H, Mazarati A, Pereira de Vasconcelos A, Wasterlain CG. Patterns of status epilepticus-induced neuronal injury during development and long-term consequences. J Neurosci. 1998;18:8382–8393. PubMed PMC

Schmued LCY, Albertson C, Slikker W., Jr Fluoro-Jade: a novel fluorochrome for the sensitive and reliable histochemical localization of neuronal degeneration. Brain Res. 1997;751:37–46. PubMed

Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998;281:1312–1316. PubMed

Turski WA, Cavalheiro EA, Schwartz M, Czuczwar SJ, Kleinrok Z, Turski L. Limbic seizures produced by pilocarpine in rats: behavioral, electroencephalographic and neuropathological study. Behav Brain Res. 1983;32:778–782. PubMed

Tuunanen J, Halonen T, Pitkanen A. Status epilepticus causes selective regional damage and loss of GABAergic neurons in the rat amygdaloid complex. Eur J Neurosci. 1996;8:2711–2725. PubMed

Tuunanen J, Lukasiuk K, Halonen T, Pitkanen A. Status epilepticus-induced neuronal damage in the rat amygdaloid complex: distribution, time-course and mechanisms. Neuroscience. 1999;94:473–495. PubMed

Vorwerk CK, Simon P, Gorla M, Katowitz W, Zurakowski D, Levin LA, Dreyer EB. Pilocarpine toxicity in retinal ganglion cells. Invest Ophthalmol Vis Sci. 1999;40:813–816. PubMed

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...