Immunomicroscopical observations on the nervous system of adult Eudiplozoon nipponicum (Monogenea: Diplozoidae)
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11403769
DOI
10.1016/s0020-7519(01)00192-8
PII: S0020-7519(01)00192-8
Knihovny.cz E-resources
- MeSH
- Biogenic Amines analysis MeSH
- Microscopy, Immunoelectron MeSH
- Immunohistochemistry MeSH
- Rabbits MeSH
- Nervous System ultrastructure MeSH
- Neuropeptides analysis MeSH
- Oligopeptides analysis MeSH
- Serotonin analysis MeSH
- Turbellaria ultrastructure MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biogenic Amines MeSH
- glycyl-tyrosyl-isoleucyl-arginyl-phenylalaninamide MeSH Browser
- Neuropeptides MeSH
- Oligopeptides MeSH
- Serotonin MeSH
Neuronal pathways have been examined in adult Eudiplozoon nipponicum (Monogenea: Diplozoidae), using cytochemistry interfaced with confocal scanning laser microscopy, in an attempt to ascertain the status of the nervous system. Peptidergic and serotoninergic innervation was demonstrated by indirect immunocytochemistry and cholinergic components by enzyme cytochemical methodology; post-embedding electron microscopical immunogold labelling revealed neuropeptide immunoreactivity at the subcellular level. All three classes of neuronal mediators were identified throughout both central and peripheral elements of a well-differentiated orthogonal nervous system. There was considerable overlap in the staining patterns for cholinergic and peptidergic components, while dual immunostaining revealed serotonin immunoreactivity to be largely confined to a separate set of neurons. The subcellular distribution of immunoreactivity to the flatworm neuropeptide, GYIRFamide, confirmed neuropeptide localisation in dense-cored vesicles in the majority of the axons and terminal varicosities of both central and peripheral nervous systems. Results reveal an extensive and chemically diverse nervous system and suggest that pairing of individuals involves fusion of central nerve elements; it is likely also that there is continuity between the peripheral nervous systems of the two partner worms.
References provided by Crossref.org
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