Aluminum-induced rapid changes in the microtubular cytoskeleton of tobacco cell lines
Language English Country Japan Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11867700
DOI
10.1093/pcp/pcf028
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Chaperonins metabolism MeSH
- Cytoskeleton drug effects metabolism MeSH
- Dinitrobenzenes pharmacology MeSH
- Microscopy, Fluorescence MeSH
- Aluminum pharmacology MeSH
- Immunoblotting MeSH
- Microtubules drug effects metabolism MeSH
- Sulfanilamides * MeSH
- Nicotiana cytology drug effects metabolism MeSH
- Tubulin drug effects metabolism MeSH
- Tyrosine metabolism MeSH
- Cell Survival drug effects MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chaperonins MeSH
- Dinitrobenzenes MeSH
- Aluminum MeSH
- oryzalin MeSH Browser
- Sulfanilamides * MeSH
- Tubulin MeSH
- Tyrosine MeSH
Aluminum (Al) is a major factor that limits plant growth in acid soils. It causes a cessation of root growth and changes in root morphology suggesting a role of the root cytoskeleton as a target of Al-toxicity. Here we report a rapid effect of Al on the microtubular cytoskeleton of the suspension tobacco cell lines BY-2 and VBI-0. Viability studies showed that the cells were more sensitive to Al during exponential phase as compared to stationary cells. During the first hours of exposure, Al induced the formation of additional bundles of cortical microtubules (cMTs), whereas the thickness of the individual bundles decreased. Prolonged exposure resulted in disorientation of cMTs. These changes of cMTs preceded the decrease of cell viability by several hours and were accompanied by an increase in the levels of alpha-tubulin (in its tyrosinated form) and elements of the tubulin-folding chaperone CCT. These findings suggest that the microtubular cytoskeleton is one of the early targets of Al toxicity.
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