Rearrangements of ribosomal DNA clusters in late generation telomerase-deficient Arabidopsis
Jazyk angličtina Země Rakousko Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
- MeSH
- anafáze genetika MeSH
- Arabidopsis genetika MeSH
- buněčné jadérko metabolismus MeSH
- chromozomy rostlin genetika metabolismus MeSH
- genová přestavba genetika MeSH
- hybridizace in situ fluorescenční MeSH
- nestabilita genomu * MeSH
- ribozomální DNA genetika MeSH
- telomerasa nedostatek MeSH
- telomery genetika metabolismus MeSH
- umělé bakteriální chromozomy MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- srovnávací studie MeSH
- Názvy látek
- ribozomální DNA MeSH
- telomerasa MeSH
The ends of eukaryotic chromosomes are capped with special nucleoprotein structures called telomeres. Telomere shortening due to telomerase inactivation may result in fusion of homologous or heterologous chromosomes, leading to their successive breakage during anaphase movement, followed by fusion of broken ends in the next cell cycle, i.e. the breakage-fusion-bridge (BFB) cycle. Using fluorescence in situ hybridization (FISH) with 25S rDNA and specific bacterial artificial chromosome (BAC) probes we demonstrate participation of chromosomes 2 and 4 of Arabidopsis thaliana AtTERT null plants in the formation of anaphase bridges. Both homologous and non-homologous chromosomes formed transient anaphase bridges whose breakage and unequal separation led to genome rearrangement, including non-reciprocal translocations and aneuploidy. The 45S rDNA regions located at the ends of chromosomes 2 and 4 were observed in chromosome bridges at a frequency approximately ten times higher than expected in the case of random fusion events. This outcome could result from a functional association of rDNA repeats at nucleoli. We also describe increased variation in the number of nucleoli in some interphase cells with supernumerary rDNA FISH signals. These data indicate that dysfunctional telomeres in Arabidopsis lead to massive genome instability, which is induced by multiple rounds of the BFB mechanism.
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Cell. 1999 May 14;97(4):527-38 PubMed
Nucleic Acids Res. 1989 Sep 25;17(18):7179-86 PubMed
Genetics. 1941 Mar;26(2):234-82 PubMed
Plant J. 2001 Apr;26(1):77-87 PubMed
Chromosome Res. 1996 Apr;4(3):207-13 PubMed
Plant Mol Biol. 1998 Jul;37(4):621-8 PubMed
Plant J. 2001 Dec;28(6):689-97 PubMed
Chromosoma. 2002 Dec;111(5):332-40 PubMed
Curr Biol. 2002 Oct 1;12 (19):1635-44 PubMed
Proc Natl Acad Sci U S A. 1939 Aug;25(8):405-16 PubMed
Trends Pharmacol Sci. 2002 Jan;23(1):1-4 PubMed
Plant J. 1996 Feb;9(2):259-72 PubMed
Nat Genet. 2002 Oct;32(2):280-4 PubMed
J Cell Biol. 1999 Feb 22;144(4):589-601 PubMed
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5357-62 PubMed
Cell. 2001 Oct 5;107(1):67-77 PubMed
Nature. 2000 Aug 10;406(6796):641-5 PubMed
Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14584-9 PubMed
Curr Opin Genet Dev. 2000 Feb;10(1):39-46 PubMed
Arabidopsis Book. 2002;1:e0083 PubMed
EMBO J. 2002 May 1;21(9):2207-19 PubMed
Nature. 1998 Apr 9;392(6676):569-74 PubMed
Plant Physiol. 1991 Jul;96(3):985-9 PubMed
Plant Mol Biol. 2002 Mar;48(4):331-7 PubMed
Protoplasma. 2001;217(4):147-53 PubMed
Mol Gen Genet. 1996 Nov 27;253(1-2):247-52 PubMed
Science. 1997 Mar 7;275(5305):1478-81 PubMed
Science. 2001 Mar 2;291(5509):1797-800 PubMed
Cell. 1997 Oct 3;91(1):25-34 PubMed
Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14813-8 PubMed
Role of alternative telomere lengthening unmasked in telomerase knock-out mutant plants
Telomeres in evolution and evolution of telomeres
Multicolor FISH mapping of the dioecious model plant, Silene latifolia
