Multicolor FISH mapping of the dioecious model plant, Silene latifolia
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- chromozomy rostlin genetika MeSH
- genetické markery genetika MeSH
- hybridizace in situ fluorescenční MeSH
- karyotypizace MeSH
- mapování chromozomů * MeSH
- pohlavní chromozomy genetika MeSH
- repetitivní sekvence nukleových kyselin genetika MeSH
- Silene genetika MeSH
- Southernův blotting MeSH
- umělé bakteriální chromozomy MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- genetické markery MeSH
Silene latifolia is a key plant model in the study of sex determination and sex chromosome evolution. Current studies have been based on genetic mapping of the sequences linked to sex chromosomes with analysis of their characters and relative positions on the X and Y chromosomes. Until recently, very few DNA sequences have been physically mapped to the sex chromosomes of S. latifolia. We have carried out multicolor fluorescent in situ hybridization (FISH) analysis of S. latifolia chromosomes based on the presence and intensity of FISH signals on individual chromosomes. We have generated new markers by constructing and screening a sample bacterial artificial chromosome (BAC) library for appropriate FISH probes. Five newly isolated BAC clones yielded discrete signals on the chromosomes: two were specific for one autosome pair and three hybridized preferentially to the sex chromosomes. We present the FISH hybridization patterns of these five BAC inserts together with previously described repetitive sequences (X-43.1, 25S rDNA and 5S rDNA) and use them to analyze the S. latifolia karyotype. The autosomes of S. latifolia are difficult to distinguish based on their relative arm lengths. Using one BAC insert and the three repetitive sequences, we have constructed a standard FISH karyotype that can be used to distinguish all autosome pairs. We also analyze the hybridization patterns of these sequences on the sex chromosomes and discuss the utility of the karyotype mapping strategy presented to study sex chromosome evolution and Y chromosome degeneration.
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Chromosoma. 1999 Aug;108(4):266-70 PubMed
Genome. 2002 Aug;45(4):745-51 PubMed
Chromosome Res. 2001;9(5):387-93 PubMed
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9454-9 PubMed
Science. 1991 Mar 1;251(4997):1030-3 PubMed
Genome. 2002 Apr;45(2):243-52 PubMed
Theor Appl Genet. 2002 Sep;105(4):491-497 PubMed
Plant J. 2001 Dec;28(6):689-97 PubMed
Genetics. 2003 Jan;163(1):321-34 PubMed
Theor Appl Genet. 2003 Aug;107(4):591-7 PubMed
Plant Cell. 2000 May;12(5):617-36 PubMed
Biotech Histochem. 1998 May;73(3):150-6 PubMed
Genetics. 2003 Feb;163(2):759-70 PubMed
Chromosome Res. 1997 Feb;5(1):57-65 PubMed
Plant Physiol. 2001 Dec;127(4):1418-24 PubMed
Genetics. 2003 Oct;165(2):935-8 PubMed
Chromosoma. 2003 Oct;112(3):116-23 PubMed
Plant Cell. 2002 Aug;14(8):1691-704 PubMed
Nucleic Acids Res. 1989 Jan 25;17(2):796 PubMed
Mol Gen Genet. 1998 Aug;259(2):133-41 PubMed
Trends Genet. 1992 Nov;8(11):372-3 PubMed
Genetics. 2002 Aug;161(4):1661-72 PubMed
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