Long term and repeated electron microscopy and PCR detection of Borrelia burgdorferi sensu lato after an antibiotic treatment
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15068199
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- Borrelia burgdorferi Group isolation & purification ultrastructure MeSH
- Time Factors MeSH
- Child MeSH
- DNA, Bacterial analysis MeSH
- Adult MeSH
- Microscopy, Immunoelectron methods MeSH
- Immunoglobulin G blood MeSH
- Immunoglobulin M blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Lyme Disease diagnosis drug therapy immunology MeSH
- Adolescent MeSH
- Polymerase Chain Reaction methods MeSH
- Blotting, Western MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- DNA, Bacterial MeSH
- Immunoglobulin G MeSH
- Immunoglobulin M MeSH
The diagnosis of Lyme disease in 18 patients has been proved by detection of Borrelia burgdorferi sensu lato when using immunoelectron microscopy or detecting its nucleic acid by PCR in the plasma or the cerebrospinal fluid. The positive results occurred in the plasma or in the cerebrospinal fluid in the period of 4-68 months after an antibiotic treatment. The typical clinical manifestations of Lyme disease were observed in 9 patients and non-specific symptoms in another 9 patients. According to presented results we can recommend repeated examination using PCR of the plasma and other biological specimens in the individuals with persistent or recurring complaints after an acute form of Lyme disease and its antibiotic treatment. Also examination of the cerebrospinal fluid with non-specific symptoms and simultaneously displayed pathology electroencephalogram and/or magnetic resonance imaging findings can be advantageous.
Metamorphoses of Lyme disease spirochetes: phenomenon of Borrelia persisters
