Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support
Language English Country Netherlands Media print
Document type Journal Article
PubMed
15110476
DOI
10.1016/j.biomaterials.2003.12.031
PII: S0142961203011803
Knihovny.cz E-resources
- MeSH
- Biodegradation, Environmental MeSH
- Biocompatible Materials chemistry MeSH
- Cell Culture Techniques methods MeSH
- Time Factors MeSH
- Sodium Chloride chemistry MeSH
- Cyclohexanols chemistry MeSH
- Nitrogen chemistry MeSH
- Microscopy, Electron MeSH
- Embryo, Mammalian cytology MeSH
- Hydrolysis MeSH
- Hydroxylamines chemistry MeSH
- Indicators and Reagents MeSH
- Stem Cells cytology MeSH
- Hydrogen-Ion Concentration MeSH
- Cells, Cultured MeSH
- Polymethacrylic Acids chemistry MeSH
- Methacrylates chemistry MeSH
- Microscopy, Electron, Scanning MeSH
- Mice MeSH
- Polyhydroxyethyl Methacrylate chemistry MeSH
- Polymers chemistry MeSH
- Cross-Linking Reagents pharmacology MeSH
- Sucrose chemistry MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Sodium Chloride MeSH
- Cyclohexanols MeSH
- Nitrogen MeSH
- ethylene dimethacrylate MeSH Browser
- Hydroxylamines MeSH
- Indicators and Reagents MeSH
- Polymethacrylic Acids MeSH
- Methacrylates MeSH
- N,O-dimethacryloylhydroxylamine MeSH Browser
- Polyhydroxyethyl Methacrylate MeSH
- Polymers MeSH
- Cross-Linking Reagents MeSH
- Sucrose MeSH
Poly(2-hydroxyethyl methacrylate) (PHEMA) crosslinked with ethylene dimethacrylate (EDMA) or N,O-dimethacryloylhydroxylamine (DMHA) was obtained in the form of slabs by bulk radical polymerization. Two porosity-inducing methods were investigated, phase separation using a low-molecular-weight porogen and a salt-leaching technique using NaCl and saccharose. Compared with the phase separation, the salt-leaching created open porous structures with voids of the size and shape of crystallites. To address its potentials in the context of stem cell therapies, undifferentiated mouse embryonic stem cells D3 (ES D3 cells) were seeded on the slabs and analyzed for the ability to grow on different types of non-degradable and/or degradable porous PHEMA hydrogels. The cells were able to proliferate only on PHEMA crosslinked with EDMA or 2 wt% DMHA. In order to assess the effect of gelatin, which is routinely used for ES cell cultures, PHEMA slabs were soaked in gelatin solutions and compared the number of cells on gelatin-treated and untreated slabs 4 days after cell seeding. Surprisingly, the number of cells was only slightly higher on gelatin-treated slabs.
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