Influence of thyroid status on the differentiation of slow and fast muscle phenotypes
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15119936
Knihovny.cz E-resources
- MeSH
- Cell Differentiation genetics MeSH
- Phenotype MeSH
- Thyroid Hormones genetics physiology MeSH
- Hyperthyroidism genetics MeSH
- Hypothyroidism genetics MeSH
- Muscle, Skeletal physiology ultrastructure MeSH
- Rats MeSH
- Rats, Inbred Lew MeSH
- Protein Isoforms genetics MeSH
- Sodium-Calcium Exchanger genetics MeSH
- Muscle Fibers, Slow-Twitch physiology MeSH
- Muscle Fibers, Fast-Twitch physiology MeSH
- Myosin Heavy Chains genetics physiology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Thyroid Hormones MeSH
- Protein Isoforms MeSH
- Sodium-Calcium Exchanger MeSH
- Myosin Heavy Chains MeSH
Muscle phenotype is determined by combined effects of intrinsic genetic and extrinsic factors like innervation, hormonal levels and mechanical factors or muscle activity. We have been studying the effect of altered thyroid hormone levels on the expression of myosin heavy chain (MyHC) isoforms in control and regenerating soleus and extensor digitorum longus muscles of euthyroid, hypothyroid or hyperthyroid female inbred Lewis rats. The fiber type composition has been determined according to the mATPase activity and immunocytochemical staining of MyHC isoforms, the content of MyHC isoforms has been determined by SDS-PAGE, the mRNA levels have been measured by RT-PCR and the ultrastructural transformation has been analyzed by electron-microscopy. Our results indicate that although the innervation plays a decisive role in the determination of muscle phenotype, levels of thyroid hormones contribute to the extent of muscle phenotype transformation.
