Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15974129
DOI
10.1016/j.chroma.2005.02.091
PII: S0021-9673(05)00761-2
Knihovny.cz E-resources
- MeSH
- Abscisic Acid analysis isolation & purification MeSH
- Indoleacetic Acids analysis isolation & purification MeSH
- Plant Growth Regulators analysis isolation & purification MeSH
- Reproducibility of Results MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Abscisic Acid MeSH
- Indoleacetic Acids MeSH
- Plant Growth Regulators MeSH
A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.
References provided by Crossref.org
Phytohormone profiling in an evolutionary framework
Can plant hormonomics be built on simple analysis? A review
Metabolic profiles of 2-oxindole-3-acetyl-amino acid conjugates differ in various plant species
Control of cytokinin and auxin homeostasis in cyanobacteria and algae
2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis
Quo vadis plant hormone analysis?
Auxin transport at cellular level: new insights supported by mathematical modelling
Functional characterization of PaLAX1, a putative auxin permease, in heterologous plant systems
