In vitro inhibitory effects of thymol and quinones of Nigella sativa seeds on cyclooxygenase-1- and -2-catalyzed prostaglandin E2 biosyntheses
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16142638
DOI
10.1055/s-2005-871288
Knihovny.cz E-resources
- MeSH
- Quinones administration & dosage pharmacology therapeutic use MeSH
- Cyclooxygenase 1 MeSH
- Cyclooxygenase 2 MeSH
- Prostaglandin-Endoperoxide Synthases metabolism MeSH
- Dinoprostone biosynthesis MeSH
- Phytotherapy * MeSH
- Inhibitory Concentration 50 MeSH
- Cyclooxygenase 2 Inhibitors MeSH
- Cyclooxygenase Inhibitors administration & dosage pharmacology therapeutic use MeSH
- Humans MeSH
- Membrane Proteins MeSH
- Nigella sativa * MeSH
- Plant Extracts administration & dosage pharmacology therapeutic use MeSH
- Seeds MeSH
- Thymol administration & dosage pharmacology therapeutic use MeSH
- Dose-Response Relationship, Drug MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Quinones MeSH
- Cyclooxygenase 1 MeSH
- Cyclooxygenase 2 MeSH
- Prostaglandin-Endoperoxide Synthases MeSH
- Dinoprostone MeSH
- Cyclooxygenase 2 Inhibitors MeSH
- Cyclooxygenase Inhibitors MeSH
- Membrane Proteins MeSH
- PTGS1 protein, human MeSH Browser
- PTGS2 protein, human MeSH Browser
- Plant Extracts MeSH
- Thymol MeSH
Dithymoquinone, thymohydroquinone, thymol and thymoquinone, compounds derived from N. sativa seeds, were investigated for their in vitro anti-inflammatory activities using cyclooxygenase-1 (COX-1) and -2 (COX-2) assays. Our results show that all substances tested possess significant inhibitory activity against at least one COX form at concentrations comparable to the active one of indomethacin. Thymol was the most active against COX-1 with an IC (50) value of 0.2 microM while thymohydroquinone and thymoquinone exhibited the strongest inhibitory effect on COX-2 with IC (50) values of 0.1 and 0.3 microM, respectively. Moreover, dithymoquinone and thymoquinone showed a limited COX-2-specific inhibition. We conclude that dithymoquinone, thymohydroquinone, thymol and thymoquinone can participate in the general anti-inflammatory activity of N. sativa and suggest that these agents should be further studied for possible use as non-steroidal anti-inflammatory drugs.
References provided by Crossref.org
