Localization, structure and polymorphism of two paralogous Xenopus laevis mitochondrial malate dehydrogenase genes
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromosomes MeSH
- Genes, Duplicate MeSH
- Expressed Sequence Tags MeSH
- Genetic Variation MeSH
- In Situ Hybridization, Fluorescence MeSH
- Introns MeSH
- Karyotyping MeSH
- Cloning, Molecular MeSH
- Conserved Sequence MeSH
- Malate Dehydrogenase chemistry genetics metabolism MeSH
- Chromosome Mapping MeSH
- Mitochondria enzymology MeSH
- Molecular Sequence Data MeSH
- Polymorphism, Genetic * MeSH
- Retroelements MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Homology, Amino Acid MeSH
- Nucleic Acid Amplification Techniques MeSH
- Xenopus laevis genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Malate Dehydrogenase MeSH
- Retroelements MeSH
Two paralogous mitochondrial malate dehydrogenase 2 (Mdh2) genes of Xenopus laevis have been cloned and sequenced, revealing 95% identity. Fluorescence in-situ hybridization (FISH) combined with tyramide amplification discriminates both genes; Mdh2a was localized into chromosome q3 and Mdh2b into chromosome q8. One kb cDNA probes detect both genes with 85% accuracy. The remaining signals were on the paralogous counterpart. Introns interrupt coding sequences at the same nucleotide as defined for mouse. Restriction polymorphism has been detected in the first intron of Mdh2a, while the individual variability in intron 6 of Mdh2b gene is represented by an insertion of incomplete retrotransposon L1Xl. Rates of nucleotide substitutions indicate that both genes are under similar evolutionary constraints. X. laevis Mdh2 genes can be used as markers for physical mapping and linkage analysis.
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