Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP-signalling pathway
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16700970
DOI
10.1017/s0967199406003546
PII: S0967199406003546
Knihovny.cz E-resources
- MeSH
- Enzyme Activation drug effects MeSH
- Aminoquinolines metabolism pharmacology MeSH
- Quinoxalines metabolism pharmacology MeSH
- Digitoxin metabolism pharmacology MeSH
- Phosphoric Diester Hydrolases metabolism MeSH
- Cyclic GMP analogs & derivatives metabolism pharmacology MeSH
- Guanylate Cyclase MeSH
- Protein Kinase Inhibitors metabolism pharmacology MeSH
- Oocytes cytology drug effects enzymology MeSH
- Oxadiazoles metabolism pharmacology MeSH
- Nitric Oxide metabolism MeSH
- Penicillamine analogs & derivatives metabolism pharmacology MeSH
- Cell Membrane Permeability drug effects MeSH
- Swine metabolism MeSH
- Cyclic GMP-Dependent Protein Kinases antagonists & inhibitors metabolism MeSH
- Receptors, Cytoplasmic and Nuclear metabolism MeSH
- Soluble Guanylyl Cyclase MeSH
- Signal Transduction * drug effects MeSH
- Nitric Oxide Synthase metabolism MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one MeSH Browser
- 6-anilino-5,8-quinolinedione MeSH Browser
- 8-bromocyclic GMP MeSH Browser
- Aminoquinolines MeSH
- Quinoxalines MeSH
- Digitoxin MeSH
- Phosphoric Diester Hydrolases MeSH
- Cyclic GMP MeSH
- Guanylate Cyclase MeSH
- Protein Kinase Inhibitors MeSH
- Oxadiazoles MeSH
- Nitric Oxide MeSH
- Penicillamine MeSH
- Cyclic GMP-Dependent Protein Kinases MeSH
- Receptors, Cytoplasmic and Nuclear MeSH
- Soluble Guanylyl Cyclase MeSH
- S-nitro-N-acetylpenicillamine MeSH Browser
- Nitric Oxide Synthase MeSH
Pig oocytes matured in vitro were parthenogenetically activated (78%) after treatment with 2 mM nitric oxide-donor (+/-)-S-nitroso-N-acetylpenicillamine (SNAP) for 24 h. Inhibition of soluble guanylyl cyclase with the specific inhibitors 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) or 6-anilino-5,8-quinolinequinone (LY83583) suppressed the SNAP-induced activation in a dose-dependent manner (23% of activated oocytes after treatment with 400 microM ODQ; 12% of activated oocytes after treatment with 40 microM LY83583). 8-Bromo-cyclic guanosine monophosphate (8-Br-cGMP), a phosphodiesterase-resistant analogue of cGMP, enhances the effect of suboptimal doses (0.1 or 0.5 mM) of the NO donor SNAP. DT3, a specific inhibitor of cGMP-dependent protein kinase (PKG, PKG), is also able to inhibit the activation of pig oocytes after NO donor treatment. Involvement of the cGMP-dependent signalling pathway is specific for NO-induced oocyte activation, because both the guanylyl cyclase inhibitor ODQ and the PKG inhibitor DT3 are unable to inhibit activation in oocytes treated with the calcium ionophore A23187. These data indicate that the activation of pig oocytes with an NO donor is cGMP-dependent and that PKG plays an important role in this mode of oocyte activation.
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