Biochemical studies of the thermal effects on DNA modifications by the antitumor cisplatin and their repair
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Antineoplastic Agents chemistry pharmacology MeSH
- Time Factors MeSH
- Cisplatin chemistry pharmacology MeSH
- DNA-Directed RNA Polymerases antagonists & inhibitors chemistry MeSH
- DNA chemistry drug effects MeSH
- HeLa Cells MeSH
- Humans MeSH
- DNA Repair * MeSH
- RNA chemical synthesis chemistry drug effects MeSH
- Cattle MeSH
- Temperature * MeSH
- Binding Sites MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antineoplastic Agents MeSH
- calf thymus DNA MeSH Browser
- Cisplatin MeSH
- DNA-Directed RNA Polymerases MeSH
- DNA MeSH
- RNA MeSH
Using biochemical methods, we have examined the effect of two factors that might play a role in the mechanism of the biological activity of cisplatin at elevated temperatures (>37 degrees C). We show that increased temperatures result in distinct alterations in the modification of the target DNA by cisplatin, and in the repair of these modifications. Our in vitro results support the view that the enhanced DNA-cross-linking efficiency of cisplatin and the lower efficiency of native DNA repair mechanisms at higher temperature play at least a partial role in the potentiation of the antitumor effects of cisplatin under conditions of mild hyperthermia.
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