Changes of endocrine function of adipose tissue in anorexia nervosa: comparison of circulating levels versus subcutaneous mRNA expression
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
17953628
DOI
10.1111/j.1365-2265.2007.02944.x
PII: CEN2944
Knihovny.cz E-zdroje
- MeSH
- adiponektin krev genetika MeSH
- antigeny diferenciační myelomonocytární genetika MeSH
- C-reaktivní protein analýza MeSH
- CD antigeny genetika MeSH
- dospělí MeSH
- exprese genu MeSH
- fyziologická adaptace MeSH
- interleukin-6 genetika MeSH
- inzulin krev MeSH
- krevní glukóza analýza MeSH
- leptinové receptory krev MeSH
- lidé MeSH
- mentální anorexie imunologie metabolismus MeSH
- messenger RNA analýza MeSH
- neparametrická statistika MeSH
- parakrinní signalizace * MeSH
- podkožní tuk chemie imunologie metabolismus MeSH
- podvýživa imunologie metabolismus MeSH
- resistin krev genetika MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- adiponektin MeSH
- antigeny diferenciační myelomonocytární MeSH
- C-reaktivní protein MeSH
- CD antigeny MeSH
- CD68 antigen, human MeSH Prohlížeč
- interleukin-6 MeSH
- inzulin MeSH
- krevní glukóza MeSH
- leptinové receptory MeSH
- messenger RNA MeSH
- resistin MeSH
OBJECTIVE: To study the influence of chronic malnutrition in patients with anorexia nervosa on endocrine function of adipose tissue on both circulating and subcutaneous fat mRNA expression level. PATIENTS AND DESIGN: A total of 12 patients with anorexia nervosa and 18 normal weight age-matched women underwent anthropometric examination, single blood drawing and subcutaneous adipose tissue biopsy. MEASUREMENTS: Serum concentrations of high-sensitive CRP (hsCRP), leptin, soluble leptin receptor, adiponectin, resistin, interleukin-6 and insulin were measured by Luminex, enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) kits. Subcutaneous adipose tissue mRNA expression of the same adipokines, adiponectin receptors 1 and 2 and immunocompetent cells marker CD68 was measured by real-time polymerase chain reaction (PCR). RESULTS: Decreased body fat content of patients with anorexia nervosa was accompanied by reduced hsCRP, leptin and increased adiponectin and soluble leptin receptor. Resistin, interleukin-6 and insulin levels did not differ from those of the control group. Fat mRNA adiponectin, leptin, interleukin-6 and CD68 expression was reduced, resistin mRNA expression was increased and adiponectin receptor 1 and 2 expression were unchanged as compared to the control group. CONCLUSIONS: Local perturbations in resistin, adiponectin and interleukin-6 mRNA expression in subcutaneous adipose tissue are not reflected by its circulating levels. These changes could be involved in some local metabolic disturbances in subcutaneous adipose tissue of anorexia nervosa patients.
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