The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Antigens, Bacterial immunology MeSH
- Bacterial Proteins immunology MeSH
- Brachyspira hyodysenteriae immunology MeSH
- Species Specificity MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Fluorescent Antibody Technique, Direct MeSH
- Gram-Negative Bacterial Infections diagnosis veterinary MeSH
- Lipoproteins immunology MeSH
- Antibodies, Monoclonal immunology MeSH
- Swine Diseases diagnosis MeSH
- Peptide Library MeSH
- Swine MeSH
- Antibodies, Bacterial immunology MeSH
- Recombinant Fusion Proteins immunology MeSH
- Antibody Specificity MeSH
- Immunoglobulin Variable Region immunology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Bacterial MeSH
- Bacterial Proteins MeSH
- Lipoproteins MeSH
- Antibodies, Monoclonal MeSH
- Peptide Library MeSH
- Antibodies, Bacterial MeSH
- Recombinant Fusion Proteins MeSH
- Immunoglobulin Variable Region MeSH
Single-chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. Recombinant Bhlp 29.7 protein was used for scFv selection and individual clones were tested by ELISA and immunofluorescent test; four unique clones were isolated. One of selected clones was able to bind specifically B. hyodysenteriae in ELISA and immunofluorescence test. This is the first report of species-specific recombinant antibodies against B. hyodysenteriae.
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