Survival of bifidobacteria administered to calves
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacteria classification isolation & purification MeSH
- Bifidobacterium drug effects metabolism physiology MeSH
- Time Factors MeSH
- DNA, Bacterial genetics MeSH
- Feces microbiology MeSH
- Phylogeny MeSH
- Gastrointestinal Tract microbiology MeSH
- In Situ Hybridization MeSH
- Microbial Sensitivity Tests MeSH
- Microbial Viability * MeSH
- Colony Count, Microbial MeSH
- Probiotics administration & dosage isolation & purification MeSH
- Cluster Analysis MeSH
- Cattle microbiology MeSH
- Bacterial Typing Techniques MeSH
- Animals MeSH
- Check Tag
- Cattle microbiology MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- DNA, Bacterial MeSH
Twenty-five bifidobacteria were isolated from feces of calves. Isolates were identified, and their functional properties and antimicrobial activity were determined. From 10 strains with suitable properties rifampicin-resistant mutants (RRBs) were prepared and mixture of RRBs was administered to 2-d-old calves. These strains were identified by sequencing as Bifidobacterium animalis ssp. animalis (6 strains), B. thermophilum (2 strains), B. choerinum (1 strain) and B. longum ssp. suis (1 strain). The control group was without probiotic treatment. Survival ability of administered bifidobacteria was monitored in fecal samples by cultivation on modified TPY agar supplemented with mupirocin, acetic acid, and rifampicin. Administered bifidobacteria survived in gastrointestinal tract of calves for at least 60 d. Other bacteria were also determined after cultivation using fluorescence in situ hybridization (FISH). Bifidobacteria and lactobacilli dominated in fecal microflora. Significantly lower amounts of E. coli and higher amounts of bifidobacteria and total anaerobes were found in the treated group relative to the control group.
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