In our previous study we applied the Agilent 44K tobacco gene chip to introduce and analyze the tobacco male gametophyte transcriptome in mature pollen and 4h pollen tubes. Here we extended our analysis post-pollen mitosis II (PMII) by including a new data set obtained from more advanced stage of the ongoing progamic phase - pollen tubes cultivated in vitro for 24 h. Pollen mitosis II marks key events in the control of male gametophyte development, the production of two sperm cells. In bicellular species covering cca 70% of angiosperms including Nicotiana tabacum, PMII takes place after pollen germination in growing pollen tube. We showed the stable and even slightly increasing complexity of tobacco male gametophyte transcriptome over long period of progamic phase-24 h of pollen tube growth. We also demonstrated the ongoing transcription activity and specific transcript accumulation in post-PMII pollen tubes cultivated in vitro. In all, we have identified 320 genes (2.2%) that were newly transcribed at least after 4h of pollen tube cultivation in vitro. Further, 699 genes (4.8%) showed over 5-fold increased accumulation after the 24h of cultivation.

Laboratory of Pollen Biology Institute of Experimental Botany Academy of Sciences of the Czech Republic Praha 6 Czech Republic

Zobrazit více v PubMed

Hafidh S, Breznenová K, Růžička P, Feciková J, Capková V, Honys D. Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis. BMC Plant Biol. 2012;12:24. doi: 10.1186/1471-2229-12-24. PubMed DOI PMC

Petrů E, Hrabětová E, Tupý J. The technique of obtaining germinating pollen without microbial contamination. Biol Plant. 1964;6:68–9. doi: 10.1007/BF02930799. DOI

Honys D, Rĕnák D, Feciková J, Jedelský PL, Nebesárová J, Dobrev P, et al. Cytoskeleton-associated large RNP complexes in tobacco male gametophyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing, and localization. J Proteome Res. 2009;8:2015–31. doi: 10.1021/pr8009897. PubMed DOI

Li C, Wong WH. Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection. Proc Natl Acad Sci U S A. 2001;98:31–6. doi: 10.1073/pnas.011404098. PubMed DOI PMC

Honys D, Twell D. Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biol. 2004;5:R85. doi: 10.1186/gb-2004-5-11-r85. PubMed DOI PMC

Pina C, Pinto F, Feijó JA, Becker JD. Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation. Plant Physiol. 2005;138:744–56. doi: 10.1104/pp.104.057935. PubMed DOI PMC

Wei LQ, Xu WY, Deng ZY, Su Z, Xue Y, Wang T. Genome-scale analysis and comparison of gene expression profiles in developing and germinated pollen in Oryza sativa. BMC Genomics. 2010;11:338. doi: 10.1186/1471-2164-11-338. PubMed DOI PMC

Qin Y, Leydon AR, Manziello A, Pandey R, Mount D, Denic S, et al. Penetration of the stigma and style elicits a novel transcriptome in pollen tubes, pointing to genes critical for growth in a pistil. PLoS Genet. 2009;5:e1000621. doi: 10.1371/journal.pgen.1000621. PubMed DOI PMC

Hafidh S, Capková V, Honys D. Safe keeping the message: mRNP complexes tweaking after transcription. Adv Exp Med Biol. 2011;722:118–36. doi: 10.1007/978-1-4614-0332-6_8. PubMed DOI

Heat stress response mechanisms in pollen development

Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics

Evolutionary history of callose synthases in terrestrial plants with emphasis on proteins involved in male gametophyte development

Characterization of pollen-expressed bZIP protein interactions and the role of ATbZIP18 in the male gametophyte

Male gametophyte development and function in angiosperms: a general concept

Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

Phosphoproteomics Profiling of Tobacco Mature Pollen and Pollen Activated in vitro

Transcriptome profiling of male gametophyte development in Nicotiana tabacum