The nuclear mRNA export receptor Mex67-Mtr2 of Trypanosoma brucei contains a unique and essential zinc finger motif
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23560737
DOI
10.1111/mmi.12217
Knihovny.cz E-resources
- MeSH
- Active Transport, Cell Nucleus * MeSH
- Nuclear Proteins chemistry genetics metabolism MeSH
- RNA, Messenger metabolism MeSH
- Nucleocytoplasmic Transport Proteins chemistry genetics metabolism MeSH
- RNA-Binding Proteins chemistry genetics metabolism MeSH
- Trypanosoma brucei brucei chemistry genetics metabolism MeSH
- Zinc Fingers * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nuclear Proteins MeSH
- RNA, Messenger MeSH
- Nucleocytoplasmic Transport Proteins MeSH
- RNA-Binding Proteins MeSH
Trypanosoma brucei is the causative agent of Human African Trypanosomiasis. Trypanosomes are early diverged protozoan parasites and show significant differences in their gene expression compared with higher eukaryotes. Due to a lack of individual gene promoters, large polycistronic transcripts are produced and individual mRNAs mature by trans-splicing and polyadenylation. In the absence of transcriptional control, regulation of gene expression occurs post-transcriptionally mainly by control of transcript stability and translation. Regulation of mRNA export from the nucleus to the cytoplasm might be an additional post-transcriptional event involved in gene regulation. However, our knowledge about mRNA export in trypanosomes is very limited. Although export factors of higher eukaryotes are reported to be conserved, only a few orthologues can be readily identified in the genome of T. brucei. Hence, biochemical approaches are needed to identify the export machinery of trypanosomes. Here, we report the functional characterization of the essential mRNA export factor TbMex67. TbMex67 contains a unique and essential N-terminal zinc finger motif. Furthermore, we could identify two interacting export factors namely TbMtr2 and the karyopherin TbIMP1. Our data show that the general heterodimeric export receptor Mex67-Mtr2 is conserved throughout the eukaryotic kingdom albeit exhibiting parasite-specific features.
References provided by Crossref.org
Trypanosomes lack a canonical EJC but possess an UPF1 dependent NMD-like pathway
Sending the message: specialized RNA export mechanisms in trypanosomes
Nuclear tRNA export in trypanosomes: a journey full of twists and turns guided by tRNA modifications
Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei
Evolution and diversification of the nuclear pore complex