Strong accumulation of chloroplast DNA in the Y chromosomes of Rumex acetosa and Silene latifolia
Language English Country Switzerland Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
24051898
DOI
10.1159/000355212
PII: 000355212
Knihovny.cz E-resources
- MeSH
- Centromere MeSH
- Chromosomes, Plant genetics MeSH
- DNA, Chloroplast * MeSH
- Species Specificity MeSH
- Genome, Chloroplast MeSH
- Gene Dosage MeSH
- In Situ Hybridization, Fluorescence MeSH
- Mutagenesis, Insertional MeSH
- Evolution, Molecular * MeSH
- Sex Chromosomes genetics MeSH
- Polymerase Chain Reaction MeSH
- Recombination, Genetic MeSH
- Rumex genetics MeSH
- Silene genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Chloroplast * MeSH
Chloroplast DNA (cpDNA) sequences are often found in plant nuclear genomes, but patterns of their chromosomal distribution are not fully understood. The distribution of cpDNA on the sex chromosomes can only be studied in dioecious plant species possessing heteromorphic sex chromosomes. We reconstructed the whole chloroplast genome of Rumex acetosa (sorrel, XY1Y2 system) from next generation sequencing data. We systematically mapped the chromosomal localization of various regions of cpDNA in R. acetosa and in Silene latifolia (white campion, XY system) using fluorescence in situ hybridization. We found that cpDNA was accumulated on the Y chromosomes of both studied species. In R. acetosa, the entire Y chromosome gathered all parts of cpDNA equally. On the contrary, in S. latifolia, the majority of the cpDNA, corresponding to the single copy regions, was localized in the centromere of the Y chromosome, while the inverted repeat region was present also in other loci. We found a stronger accumulation of cpDNA on the more degenerated Y1 and Y2 chromosomes of R. acetosa than in evolutionary younger S. latifolia Y chromosome. Our data stressed the prominent role of the Y chromosome centromere in cpDNA accumulation.
References provided by Crossref.org
Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa
Impact of Repetitive Elements on the Y Chromosome Formation in Plants
Impact of repetitive DNA on sex chromosome evolution in plants
Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia