Effects of sphingomyelin/ceramide ratio on the permeability and microstructure of model stratum corneum lipid membranes
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24824073
DOI
10.1016/j.bbamem.2014.05.001
PII: S0005-2736(14)00173-4
Knihovny.cz E-resources
- Keywords
- Ceramide, Lipid membrane, Permeability, Sphingomyelin, Stratum corneum model, X-ray diffraction,
- MeSH
- Ceramides metabolism MeSH
- Cholesterol chemistry metabolism MeSH
- X-Ray Diffraction MeSH
- Epidermis metabolism MeSH
- Skin chemistry metabolism MeSH
- Fatty Acids, Nonesterified chemistry metabolism MeSH
- Humans MeSH
- Lipid Bilayers metabolism MeSH
- Membrane Lipids chemistry MeSH
- Cell Membrane Permeability MeSH
- Sphingomyelins metabolism MeSH
- Chromatography, High Pressure Liquid MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ceramides MeSH
- Cholesterol MeSH
- Fatty Acids, Nonesterified MeSH
- Lipid Bilayers MeSH
- Membrane Lipids MeSH
- Sphingomyelins MeSH
The conversion of sphingomyelin (SM) to a ceramide (Cer) by acid sphingomyelinase (aSMase) is an important event in skin barrier development. A deficiency in aSMase in diseases such as Niemann-Pick disease and atopic dermatitis coincides with impaired skin barrier recovery after disruption. We studied how an increased SM/Cer ratio influences the barrier function and microstructure of model stratum corneum (SC) lipid membranes. In the membranes composed of isolated human SC Cer (hCer)/cholesterol/free fatty acids/cholesteryl sulfate, partial or full replacement of hCer by SM increased water loss. Partial replacement of 25% and 50% of hCer by SM also increased the membrane permeability to theophylline and alternating electric current, while a higher SM content either did not alter or even decreased the membrane permeability. In contrast, in a simple membrane model with only one type of Cer (nonhydroxyacyl sphingosine, CerNS), an increased SM/Cer ratio provided a similar or better barrier against the permeation of various markers. X-ray powder diffraction revealed that the replacement of hCer by SM interferes with the formation of the long periodicity lamellar phase with a repeat distance of d=12.7nm. Our results suggest that SM-to-Cer processing in the human epidermis is essential for preventing excessive water loss, while the permeability barrier to exogenous compounds is less sensitive to the presence of sphingomyelin.
References provided by Crossref.org
Lysosphingolipids in ceramide-deficient skin lipid models
Polymorphism, Nanostructures, and Barrier Properties of Ceramide-Based Lipid Films
Behavior of 1-Deoxy-, 3-Deoxy- and N-Methyl-Ceramides in Skin Barrier Lipid Models
