Proteomic analysis of barley cell nuclei purified by flow sorting
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25059295
DOI
10.1159/000365311
PII: 000365311
Knihovny.cz E-resources
- MeSH
- Cell Nucleus genetics MeSH
- Interphase genetics MeSH
- Hordeum genetics MeSH
- Proteome genetics MeSH
- Proteomics methods MeSH
- Flow Cytometry methods MeSH
- Plant Proteins genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Proteome MeSH
- Plant Proteins MeSH
Many proteins are present in the nucleus; some are involved with its structural and functional organization, some with gene expression, and some with cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both the artifactual alteration of the proteins and the extent of contamination with non-nuclear proteins. The conventional multi-step fractionation procedure is both laborious and prone to contamination. Here, we describe a single-step method based on flow sorting. The method allows the separation of G1, S and G2 phase nuclei and minimizes the risk of contamination by non-nuclear proteins. Preliminary results obtained using G1 phase cell nuclei from barley root tips indicate that flow sorting coupled with a protein/peptide separation and mass spectrometry will permit a comprehensive characterization of the plant nuclear proteome.
References provided by Crossref.org
Auxin Metabolite Profiling in Isolated and Intact Plant Nuclei
Molecular Characterization of Novel x-Type HMW Glutenin Subunit 1B × 6.5 in Wheat
Proteome Analysis of Condensed Barley Mitotic Chromosomes
Chromosome analysis and sorting
Auxins and Cytokinins-The Role of Subcellular Organization on Homeostasis
