The use of FM dyes to analyze plant endocytosis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Arabidopsis cytology growth & development MeSH
- Staining and Labeling MeSH
- Cell Tracking MeSH
- Endocytosis genetics MeSH
- Phenylurea Compounds chemistry MeSH
- Fluorescent Dyes chemistry MeSH
- Microscopy, Fluorescence MeSH
- Plant Roots cytology growth & development MeSH
- Molecular Biology methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alpha-methylbenzyl-2-fluoro-4-methylphenylurea MeSH Browser
- Phenylurea Compounds MeSH
- Fluorescent Dyes MeSH
FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes. Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery.
References provided by Crossref.org
NtGNL1a ARF-GEF acts in endocytosis in tobacco cells
