Comparison of periprosthetic tissues in knee and hip joints: differential expression of CCL3 and DC-STAMP in total knee and hip arthroplasty and similar cytokine profiles in primary knee and hip osteoarthritis
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
25151085
DOI
10.1016/j.joca.2014.08.004
PII: S1063-4584(14)01216-3
Knihovny.cz E-zdroje
- Klíčová slova
- Aseptic loosening, Cytokines, Gene expression profile, Osteoarthritis, Total hip arthroplasty, Total knee arthroplasty,
- MeSH
- adaptorové proteiny signální transdukční genetika metabolismus MeSH
- artroplastiky kloubů metody MeSH
- artróza kolenních kloubů genetika metabolismus chirurgie MeSH
- artróza kyčelních kloubů genetika metabolismus chirurgie MeSH
- chemokin CCL3 biosyntéza genetika MeSH
- cytokiny biosyntéza genetika MeSH
- imunohistochemie MeSH
- kolenní kloub metabolismus chirurgie MeSH
- kyčelní kloub metabolismus chirurgie MeSH
- lidé středního věku MeSH
- lidé MeSH
- membránové proteiny genetika metabolismus MeSH
- messenger RNA genetika MeSH
- náhrada kyčelního kloubu MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- regulace genové exprese * MeSH
- retrospektivní studie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- totální endoprotéza kolene MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- adaptorové proteiny signální transdukční MeSH
- CCL3 protein, human MeSH Prohlížeč
- chemokin CCL3 MeSH
- cytokiny MeSH
- DCSTAMP protein, human MeSH Prohlížeč
- membránové proteiny MeSH
- messenger RNA MeSH
OBJECTIVE: To identify expression profiles (EP) associated with aseptic loosening of total knee arthroplasty (TKA) and to compare them with EP observed in total hip arthroplasty (THA), and primary knee and hip osteoarthritis (OA). DESIGN: Gene EP of TNF, IL-6, IL-8, CHIT1, BMP4, CCL3, CCL18, MMP9, RANKL, OPG, DC-STAMP and SOCS3 were assessed using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) on tissues retrieved from patients with aseptically failed TKA (n = 21), THA (n = 41) and primary knee (n = 20) and hip (n = 17) OA. Immunohistochemistry was applied to localize the proteins. RESULTS: When compared to knee OA, the pseudosynovial tissue in TKA exhibit (1) elevation of alternative macrophage activation marker (CHIT1), chemokine (IL-8), and a proteolytic enzyme (MMP9); (2) downregulation of pro-inflammatory cytokine (TNF), osteoclastic regulator (OPG) and a stimulator of bone formation (BMP4); (3) no difference in IL-6, CCL3, CCL18, RANKL, DC-STAMP and SOCS3. The EP in TKA differed from EP in aseptically failed THA by lower CCL3 and DC-STAMP mRNA and protein expression. EP of all studied inflammatory and osteoclastogenic molecules were similar in knee and hip OA. CONCLUSIONS: Comparing to OA, aseptic loosening of TKA is associated with upregulated expression of CHIT1, IL-8 and MMP9, dysregulated RANKL:OPG ratio and low levels of inflammatory cytokines. Similar cytokine profiles were associated with primary knee and hip OA. Further research is required to explain the differences in CCL3 and DC-STAMP expression between failed TKA and THA.
Department of Immunology Faculty of Medicine and Dentistry Palacky University Olomouc Czech Republic
Citace poskytuje Crossref.org
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