Comparison of periprosthetic tissues in knee and hip joints: differential expression of CCL3 and DC-STAMP in total knee and hip arthroplasty and similar cytokine profiles in primary knee and hip osteoarthritis
Language English Country Great Britain, England Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
25151085
DOI
10.1016/j.joca.2014.08.004
PII: S1063-4584(14)01216-3
Knihovny.cz E-resources
- Keywords
- Aseptic loosening, Cytokines, Gene expression profile, Osteoarthritis, Total hip arthroplasty, Total knee arthroplasty,
- MeSH
- Adaptor Proteins, Signal Transducing genetics metabolism MeSH
- Arthroplasty, Replacement methods MeSH
- Osteoarthritis, Knee genetics metabolism surgery MeSH
- Osteoarthritis, Hip genetics metabolism surgery MeSH
- Chemokine CCL3 biosynthesis genetics MeSH
- Cytokines biosynthesis genetics MeSH
- Immunohistochemistry MeSH
- Knee Joint metabolism surgery MeSH
- Hip Joint metabolism surgery MeSH
- Middle Aged MeSH
- Humans MeSH
- Membrane Proteins genetics metabolism MeSH
- RNA, Messenger genetics MeSH
- Arthroplasty, Replacement, Hip MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Gene Expression Regulation * MeSH
- Retrospective Studies MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Arthroplasty, Replacement, Knee MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Adaptor Proteins, Signal Transducing MeSH
- CCL3 protein, human MeSH Browser
- Chemokine CCL3 MeSH
- Cytokines MeSH
- DCSTAMP protein, human MeSH Browser
- Membrane Proteins MeSH
- RNA, Messenger MeSH
OBJECTIVE: To identify expression profiles (EP) associated with aseptic loosening of total knee arthroplasty (TKA) and to compare them with EP observed in total hip arthroplasty (THA), and primary knee and hip osteoarthritis (OA). DESIGN: Gene EP of TNF, IL-6, IL-8, CHIT1, BMP4, CCL3, CCL18, MMP9, RANKL, OPG, DC-STAMP and SOCS3 were assessed using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) on tissues retrieved from patients with aseptically failed TKA (n = 21), THA (n = 41) and primary knee (n = 20) and hip (n = 17) OA. Immunohistochemistry was applied to localize the proteins. RESULTS: When compared to knee OA, the pseudosynovial tissue in TKA exhibit (1) elevation of alternative macrophage activation marker (CHIT1), chemokine (IL-8), and a proteolytic enzyme (MMP9); (2) downregulation of pro-inflammatory cytokine (TNF), osteoclastic regulator (OPG) and a stimulator of bone formation (BMP4); (3) no difference in IL-6, CCL3, CCL18, RANKL, DC-STAMP and SOCS3. The EP in TKA differed from EP in aseptically failed THA by lower CCL3 and DC-STAMP mRNA and protein expression. EP of all studied inflammatory and osteoclastogenic molecules were similar in knee and hip OA. CONCLUSIONS: Comparing to OA, aseptic loosening of TKA is associated with upregulated expression of CHIT1, IL-8 and MMP9, dysregulated RANKL:OPG ratio and low levels of inflammatory cytokines. Similar cytokine profiles were associated with primary knee and hip OA. Further research is required to explain the differences in CCL3 and DC-STAMP expression between failed TKA and THA.
Department of Immunology Faculty of Medicine and Dentistry Palacky University Olomouc Czech Republic
References provided by Crossref.org
Diagnosis and management of implant debris-associated inflammation
Inflammation time-axis in aseptic loosening of total knee arthroplasty: A preliminary study
Cross-Disease Innate Gene Signature: Emerging Diversity and Abundance in RA Comparing to SLE and SSc
