Synthesis of base-modified 2'-deoxyribonucleoside triphosphates and their use in enzymatic synthesis of modified DNA for applications in bioanalysis and chemical biology
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25321948
DOI
10.1021/jo5020799
Knihovny.cz E-resources
- MeSH
- Biochemical Phenomena MeSH
- Deoxyribonucleosides chemical synthesis chemistry MeSH
- DNA-Directed DNA Polymerase chemistry metabolism MeSH
- DNA chemistry metabolism MeSH
- Phosphorylation MeSH
- Molecular Structure MeSH
- Nucleosides chemical synthesis chemistry MeSH
- Nucleotides chemical synthesis chemistry MeSH
- Oligonucleotides chemistry MeSH
- Base Sequence MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Deoxyribonucleosides MeSH
- DNA-Directed DNA Polymerase MeSH
- DNA MeSH
- Nucleosides MeSH
- Nucleotides MeSH
- Oligonucleotides MeSH
The synthesis of 2'-deoxyribonucleoside triphosphates (dNTPs) either by classical triphosphorylation of nucleosides or by aqueous cross-coupling reactions of halogenated dNTPs is discussed. Different enzymatic methods for synthesis of modified oligonucleotides and DNA by polymerase incorporation of modified nucleotides are summarized, and the applications in redox or fluorescent labeling, as well as in bioconjugations and modulation of interactions of DNA with proteins, are outlined.
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