Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made.

Department of Microbiology Faculty of Medicine and St Anne's Faculty Hospital Brno 65691 Czech Republic

Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic v v i Královopolská 147 Brno 61264 Czech Republic

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