Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.

Centre for Material Research Brno University of Technology Purkyňova 118 Brno 61200 Czech Republic

Department of Microbiology Faculty of Medicine and St Anne's Faculty Hospital Brno 65691 Czech Republic

Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic v v i Královopolská 147 Brno 61264 Czech Republic

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