Arabidopsis proteome responses to the smoke-derived growth regulator karrikin
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25746380
DOI
10.1016/j.jprot.2015.02.011
PII: S1874-3919(15)00075-5
Knihovny.cz E-resources
- Keywords
- Butenolide, Growth regulators, Karrikin, Proteome, Smoke,
- MeSH
- Arabidopsis drug effects metabolism MeSH
- Models, Biological * MeSH
- Chloroplasts drug effects metabolism MeSH
- Furans chemistry pharmacology MeSH
- Smoke MeSH
- Protein Interaction Mapping methods MeSH
- Computer Simulation MeSH
- Proteome metabolism MeSH
- Pyrans chemistry pharmacology MeSH
- Gene Expression Regulation, Plant drug effects physiology MeSH
- Plant Growth Regulators metabolism MeSH
- Plant Proteins metabolism MeSH
- Signal Transduction drug effects physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3-methyl-2H-furo(2,3-c)pyran-2-one MeSH Browser
- Furans MeSH
- Smoke MeSH
- Proteome MeSH
- Pyrans MeSH
- Plant Growth Regulators MeSH
- Plant Proteins MeSH
UNLABELLED: Karrikins are butenolide plant growth regulators in smoke from burning plant material that have proven ability to promote germination and seedling photomorphogenesis. However, the molecular mechanisms underlying these processes are unclear. Here we provide the first proteome-wide analysis of early responses to karrikin in plants (Arabidopsis seedlings). Image analysis of two-dimensionally separated proteins, Rubisco-depleted proteomes and phosphoproteomes, together with LC-MS profiling, detected >1900 proteins, 113 of which responded to karrikin treatment. All the differentially abundant proteins (except HSP70-3) are novel karrikin-responders, and most are involved in photosynthesis, carbohydrate metabolism, redox homeostasis, transcription control, proteosynthesis, protein transport and processing, or protein degradation. Our data provide functionally complementary information to previous identifications of karrikin-responsive genes and evidence for a novel karrikin signalling pathway originating in chloroplasts. We present an updated model of karrikin signalling that integrates proteomic data and is supported by growth response observations. BIOLOGICAL SIGNIFICANCE: Karrikin has shown promising potential in agricultural applications, yet this process is poorly understood at the molecular level. To the best of our knowledge, this is the first survey of early global proteomic responses to karrikin in plants (Arabidopsis seedlings). The combination of label-free LC-MS profiling and 2-DE analyses provided highly sensitive snapshots of protein abundance and quantitative information on proteoform-level changes. These results present evidence of proteasome-independent karrikin signalling pathways and provide novel targets for detailed mechanistic studies using, e.g., mutants and transgenic plants.
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